Abstract Enzymatic browning causes quality losses in the soybean sprout industry. Herein, effects of harpin, a proteinaceous bacterial elicitor isolated from Erwinia amylovora , regulating enzymatic and antioxidant nutrient accumulation stored were investigated. Enhanced growth (evaluated by length fresh weight) occurred after spraying three times (0, 2, 4 days sowing) with 30 mg L −1 harpin du...

Ethanolamine ammonia-lyase is a bacterial enzyme that catalyzes the adenosylcobalamin-dependent conversion of certain vicinal amino alcohols to oxo compounds and ammonia. Studies of ethanolamine ammonia-lyase from Clostridium sp. and Escherichia coli have suggested that the enzyme is a heterodimer composed of subunits of Mr approximately 55,000 and 35,000. Using a partial Sau3A Salmonella typhi...

Abstract Phenolic acids are the major secondary metabolites and significant bioactive constituents of medicinal plant Salvia miltiorrhiza. Many enzyme-encoding genes transcription factors involved in biosynthesis phenolic have been identified, but underlying post-translational regulatory mechanisms poorly understood. Here, we demonstrate that S. miltiorrhiza Kelch repeat F-box protein SmKFB5 ph...

Abstract The cereal aphids Rhopalosiphum padi L. are serious pests on grain crops, reducing the quality and yield by direct feeding damage virus transmission. changes in physiological biochemical parameters of two wheat cultivars under aphid stress were investigated to understand aphid-resistance mechanisms. activity levels phenylalanine ammonia-lyase (PAL), peroxidase (POD), tyrosine (TAL), Ca...

Aromatic amino acid ammonia-lyases and aromatic amino acid 2,3-aminomutases contain the post-translationally formed prosthetic 3,5-dihydro-4-methylidene-5H-imidazol-5-one (MIO) group. MIO enzymes catalyze the stereoselective synthesis of α- or β-amino acid enantiomers, making these chemical processes environmentally friendly and affordable. Characterization of novel inhibitors enables structura...

PAHdb, a legacy of and resource in genetics, is a relational locus-specific database (http://www.pahdb.mcgill.ca). It records and annotates both pathogenic alleles (n = 439, putative disease-causing) and benign alleles (n = 41, putative untranslated polymorphisms) at the human phenylalanine hydroxylase locus (symbol PAH). Human alleles named by nucleotide number (systematic names) and their tri...

The dehydroalanine present in histidine ammonia lyase is essential for the activity of this enzyme, and may be at the active site. This was shown by recovery of 3H-alanine4 and loss of activity on reduction of the enzyme with NaBaH4, and by protection of both reduction and inactivation by histidine. Since several pyruvate-containing enzymes have been described recently (1P17), it is important t...

Phenylketonuria (PKU) is a genetic metabolic disease in which the decrease or loss of phenylalanine hydroxylase (PAH) activity results in elevated, neurotoxic levels of phenylalanine (Phe). Due to many obstacles, PAH enzyme replacement therapy is not currently an option. Treatment of PKU with an alternative enzyme, phenylalanine ammonia lyase (PAL), was first proposed in the 1970s. However, iss...

aril browning threatens production, consumption, and exports of pomegranates, because affected fruit cannot be externally distinguished from healthy fruit. this study compared the mineral, biochemical composition, and related enzyme activities in affected brown arils with healthy ones in ‘malase saveh’ pomegranates. the results indicated that concentrations of cu in the aril and k, mg, and mn i...

Aryl metabolite biosynthesis was studied in the white rot fungus Bjerkandera adusta cultivated in a liquid medium supplemented with L-phenylalanine. Aromatic compounds were analyzed by gas chromatography-mass spectrometry following addition of labelled precursors ((14)C- and (13)C-labelled L-phenylalanine), which did not interfere with fungal metabolism. The major aromatic compounds identified ...