Epigenetic inheritance was transmitted through selection over five generations of extreme early, but not late flowering time phenotypic lines in Fragaria vesca. Epigenetic variation was initially artificially induced using the DNA demethylation reagent 5-azacytidine (5-azaC). It is the first report to explore epigenetic variant selection and phenotypic trait inheritance in strawberry. Transmiss...

The study of adaptive genetic variation in natural populations is central to evolutionary biology. Quantitative genetics methods, however, are hardly applicable to long-lived organisms, and current knowledge on adaptive genetic variation in wild plants mostly refers to annuals and short-lived perennials. Studies on long-lived species are essential to explore possible life-history correlates of ...

With increasing incidence of multidrug-resistant tuberculosis (MDR-TB), accurate drug susceptibility testing (DST) of Mycobacterium tuberculosis to first-line drugs has become crucial for proper patient management. We evaluated concordance of DST results for 70 M. tuberculosis isolates across two phenotypic and two molecular methods: BACTEC 460TB, MGIT 960 system, GenoType MTBDRplus and DNA seq...

Among these methods emerged recently, data reduction approaches (a constructive induction strategy) such as the multifactor dimensionality reduction method (MDR) [36,37], the combinatorial partitioning method [38], and the restricted partition method [39], are promising to address the multidimensionality problems. Rather than modeling the interaction term per se as with regression methods, a da...

Conventional phenotypic drug susceptibility testing (DST) methods for Mycobacterium tuberculosis are laborious and very time-consuming. Early detection of drug-resistant tuberculosis (TB) is essential for prevention and control of TB transmission. We have developed a pyrosequencing method for simultaneous detection of mutations associated with resistance to rifampin, isoniazid, ethambutol, amik...

this study aims at molecular identification of salmonella infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. a total of 46 salmonella-suspected samples isolated from backyard chickens of northern iran were collected. serotyping was done by the traditional method and then confirmed by pcr. antimicrobial susceptibility of the isolates against 13 antim...