Monocytes from blood and from spleen have been cultivated in fluid medium in Carrel flasks for over 2 months. Diluted serum supplied all the essential nutritive substances. Cultivation in fluid was made possible by adjusting the initial pH of the fluid to 7.4, and not allowing it to fall below 7.0 or 6.8. The cells remained in good condition when the pH was adjusted with either lactic acid, hyd...

A biocompatible and functional interface can improve the sensitivity of bioelectronics. Here, 3-aminopropyl trimethoxysilane (APTMS) and 3-mercaptopropyl trimethoxysilane (MPTMS) self-assembled monolayers (SAMs) were independently modified on the surface of silicon nanowire metal-oxide-semiconductor field effect transistors (NW-MOSFETs). Those SAMs-modified silicon NW-MOSFETs were used to discr...

Previous work showed that the exocyclic amino groups of nucleic acid components react quickly at ambient temperature with acetaldehyde and ethanol to yield mixed acetals [R-NH-CH(CH3)-O-C2H5]. We now find that the same type of reaction occurs readily with the nitrogen of 3-substituted indoles (e.g., indole-3-acetic acid and N-acetyltryptophan), analogues of the amino acid tryptophan. In contras...

The kinetics of the oxidation-reduction reactions between horse heart cytochrome c, Euglena gracilis cytochrome c552, and ions (ascorbate, ferricyanide, and ferrocyanide) was investigated as a function of ionic strength at pH 7, 25 degrees C. The ionic strength was varied between 0.002 and 0.02 M. Data were analyzed according to four different functions of ionic strength. Results showed that th...

Fe3O4-containing cotton-like mesoporous silica system (Fe3O4@NCSM) was studied with regard to its performance towards the photodecolorization of bromothymol blue (BTB) aqueous solution. The surface properties of the functionalized catalyst were analyzed by a series of characterization techniques like FTIR, XRD, BET, SEM and TEM. The N2 Adso...

Fe3O4-containing cotton-like mesoporous silica system (Fe3O4@NCSM) was studied with regard to its performance towards the photodecolorization of bromothymol blue (BTB) aqueous solution. The surface properties of the functionalized catalyst were analyzed by a series of characterization techniques like FTIR, XRD, BET, SEM and TEM. The N2 Adso...

background gastroesophageal reflux disease (gerd) is a common problem seen in pediatrics and adolescents. the bravo tm ph monitoring system is a capsule that detects and quantifies the severity of reflux disease. this test is also valuable for evaluation of patients with extra-gastrointestinal manifestations of gerd such as asthma, chronic cough, hoarseness, aspiration pneumonia, and pre/post f...

The highly regulated pH of cells and the less-regulated pH of the surrounding extracellular matrix (ECM) is the result of a delicate balance between metabolic processes and proton production, proton transportation, chemical buffering, and vascular removal of waste products. Malignant cells show a pronounced increase in metabolic processes where the 10- to 15-fold rise in glucose consumption is ...