Ultrasensitive photoelectrochemical immunoassay of polycyclic aromatic hydrocarbon (PAH) is proposed using an antibody-modified nanostructured TiO(2) electrode combined with bifunctional gold nanoparticles modified with the PAH antigen and horseradish peroxidase (HRP). The HRP-catalyzed reaction prompts the electron transfer between the electrode and electrolyte causing an excellent photocataly...

Submit Manuscript | http://medcraveonline.com Abbreviations: SPR: Surface plasmon resonance; ISFET: Ion Sensitive Field Effect Transistor; EnFET: Enzyme Field Effect Transistor; μTAS: Micro Total Analysis System; HCMV: Human Cytomegalovirus; ELISA: Enzyme-Linked Immunosorbent Assay; CNC: Computer Numerical Control; CAD: Computer Assisted Design; BSA: Bovine Serum Albumin; HPAb: Human Polyclonal...

We describe a gentamicin assay in which a peroxidase-gentamicin conjugate competes with gentamicin for binding to a gentamicin antibody adsorbed to a polystyrene solid phase. The assay can be completed in 30 min and requires 50 microliter of diluted serum. The precision and accuracy are equivalent to that of a radioimmunoassay technique and the reagents are stable for several months.

A new label-free chemiluminescence (CL) immunoassay method is proposed for the cheap, convenient and sensitive detection of proteins through the co-immobilization of a capture antibody and horseradish peroxidase on a sensing interface. The specific immunoreaction on the interface effectively inhibits the enzymatic CL reaction, thus causing a decrease in CL signals.

Abbreviations: Ab, antibody; ALP, alkaline phospha atrazine conjugated with horseradish peroxidase; CE, c nanotubes; CV, cyclic voltammetry; ELISA, enzyme-linke high-density polyethylene; HRP, horseradish peroxid magnetic beads; NP, nanoparticle; PAP, p-aminoph phosphate; PET, polyethylene terephthalate; POC, point electrode; RT, room temperature; TMB, 3,3′,5,5′-tetramet tetramethylbenzidine di...

With a view to improving the sensitivity of serological detection of Aspergillus galactomannan (GM), a europium-linked time-resolved fluoroimmunoassay was developed. This method was compared to an enzyme-linked immunosorbent assay using a peroxidase-conjugated detector antibody. No increase in the sensitivity of the detection of GM standards was seen with the europium-based fluoroimmunoassay.