This research examined four factors on the fermentation of xylose by Candida shehatae, and the following conclusions were reached: (1) A minimal medium is effective for producing ethanol. (2) Peptone and casamino acids stimulate ethanol production. (3) Aeration is important inobtaining good ethanol production rates and yields. (4) The maximal rate ofethanol production is attained around pH 3.2-...

The N-unregulated white rot fungus Bjerkandera sp. strain BOS55 was cultured in 1 liter of peptone-yeast extract medium to produce lignin peroxidase (LiP). During the LiP assay, the oxidation of veratryl alcohol to veratraldehyde was inhibited due to tyrosine present in the peptone and the yeast extract.

The Arizona group of paracolon bacteria was first investigated by Peluffo et al. (1942). Studies on this group of organisms finally culminated in the report by Edwaards et al. (1947) that established an antigenic scheme of classification independent of the Salmonella scheme. The BethesdaBallerup group of paracolons was definitively studied by West and Edwards (1954), although the original Balle...

Frazier (1926) described a plate method of testing for gelatine liquefaction. The gelatine was incorporated in a low-peptone agar medium. After growing the organism on the medium, the occurrence of digestion was shown by treating the plates with acid mercuric chloride or tannic acid. Frazier claimed that his method was more sensitive and gave quicker results than the usual gelatine " stab" meth...

Synchronization of cell division in a variety of microorganisms has been achieved by temperature shifts or by starvation. Two reports exist which mention svnchronization of the budding process in yeast by starvation (Beam et al., 1954; Ogur et al., 1953). In both cases, the demonstration of synchronization was incidental to the primary purpose of the experiments performed, and was therefore not...

1. Injection of a solution of peptone directly into the artery of the leg of a dog is followed by an abrupt increase in leg volume. This is of short duration and is quickly followed, usually in less than (1/2) minute, by a decrease in the volume of the limb below that previous to the injection. 2. The phases of the leg volume curve bear a fairly definite relation to the changes in arterial pres...

Nine strains of bacteroides fragilis were cultivated in stirred fermentors and tested for their ability to produce glycosidases. B. fragilis subsp. vulgatus B70 was used for optimizing the production of glycosidases. The highest bacterial yield was obtained in proteose peptone-yeast extract medium. The optimum pH for maximal bacterial yield was 7.0, and the optimum temperature for growth was 37...

Statistical analyses were applied to optimize the medium composition for the mycelial growth and polysaccharide production by Tremella aurantialba in shake flask cultures. Firstly, four significant factors (xylan, peptone, wheat bran and corn powder) on mycelial growth and polysaccharide yield (p£0.05) were obtained using one-at-a-time design. Subsequently, in order to study the mutual interact...

A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volume (P/V) and constant tip speed. Moreover, lipase immobilization on different materials was also ...

The growth of yeast cells in batches in glucose-based media is a standard condition in most yeast laboratories. Most gene expression experiments are done by taking this condition as a reference. Presumably, cells are in a stable physiological condition that can be easily reproduced in other laboratories. With this assumption, however, it is necessary to consider that the average amount of the m...