The deprotection step is crucial in order to secure a good quality product in Fmoc solid phase peptide synthesis. 9-Fluorenylmethoxycarbonyl (Fmoc) removal is achieved by a two-step mechanism reaction favored by the use of cyclic secondary amines; however, the efficiency of the reaction could be affected by side reactions and by-product formation. Several aspects have to be taken into considera...

The effect of antibiotics and other agents on-peptide bond synthesis by mammalian polyribosomes was determined by studying peptidyl-puromycin synthesis. Sparsomycin, anisomycin, cycloheximide, gougerotin, blasticidin S, and cryptopleurine inhibited the reaction. Sparsomycin and anisomytin inhibited the peptidyl-puromycin synthesis competitively with respect to puromycin. Arabinose analogs of pu...

The effect of antibiotics and other agents on-peptide bond synthesis by mammalian polyribosomes was determined by studying peptidyl-puromycin synthesis. Sparsomycin, anisomycin, cycloheximide, gougerotin, blasticidin S, and cryptopleurine inhibited the reaction. Sparsomycin and anisomytin inhibited the peptidyl-puromycin synthesis competitively with respect to puromycin. Arabinose analogs of pu...

Lanthanide(III) ions can accelerate the hydrolysis of phosphomonoesters and phosphodiesters in neutral aqueous solution. In this paper, lanthanide-mediated dephosphorylation has been applied in aqueous media as an orthogonal cleavage condition that can be employed in conventional solid phase peptide synthesis (SPPS). A phosphorylated polymeric support for SPPS was developed using Boc chemistry....

We present here a new, general, solid phase strategy for the synthesis of sequence independent peptidyl-fluoromethyl ketones using standard Fmoc peptide chemistry. Our method is based on the synthesis of bifunctional linkers which allows the incorporation of amino acid fluoromethyl ketone unit at the C-terminal end of peptide sequences. Application of this approach for the synthesis of activity...