Phenylboronic acid-modified PEI was prepared by the reaction of 1800 Da PEI with 4-(bromomethyl)phenylboronic acid. It is much more effective than unmodified PEI for gene delivery. The covalently incorporated boronic acid groups achieve the greatly enhanced gene delivery efficiency partially through improving condensation ability to DNA, and partially through facilitating cell uptake due to int...

Poly(ethylenimine) (PEI) is a synthetic polycation that has been used successfully for gene delivery both in vitro and in vivo due, in theory, to a form of protection that is afforded to the carried plasmids. In this study the stability of PEI/DNA complexes was demonstrated using deoxyribonuclease (DNase) 1 and DNase 2, various levels of pH, and increasing exposure times. DNA that was complexed...

Percutaneous ethanol injection (PEI) was first introduced for treatment of thyroid nodules in 1990 (1). The procedure has previously been used for treatment of several benign and malignant conditions, including parathyroid and hepatic tumors (2). Özdemir and co-workers were the first researchers who performed PEI in Turkey with successful results (3). This paper reports our center’s initial exp...

A polymer-based gene delivery system, denoted as “DNA/polycation/polyanion”, is designed based on the concept of polyelectrolyte multilayer fabrication by the layer-by-layer polyelectrolyte self-assembly technology. The comparisons of the physicochemical properties between ‘core’ DNA/branched polyethylenimine (PEI) and DNA/chitosan complexes suggest that DNA/PEI complexes form as colloidally st...

In this study, calcium carbonate (CaCO3) nanoparticles with spherical structure were regulated by arginine and successfully synthesized via a facile co-precipitation method. The average particle size of as-prepared CaCO3 was about 900 nm. The properties of nanostructured CaCO3 particles were characterized by scanning electron microscope, Fourier transform infrared spectroscopy, X-ray diffractio...

For improving the therapeutic efficiency of tumors and decreasing undesirable side effects, ternary complexes were developed by coating pH-sensitive PEG-b-PLL-g-succinylsulfathiazole (hereafter abbreviated as PPSD) with DNA/PEI polyplexes via electrostatic interaction. PPSD‘can efficiently shield the surface charge of DNA/PEI. The gene transfection efficiency of ternary complexes was lower than...

The recognition of phosphatidylserine on the erythrocyte membrane mediates erythrophagocytosis by resident spleen macrophages. The application of phosphatidylserine to a gene vector may be a novel approach for splenic drug delivery. Therefore, we chose 1,2-dioleoyl-sn-glycero-3-phospho-L-serin (DOPS) as an analogue of phosphatidylserine for splenic gene delivery of plasmid DNA (pDNA). In the pr...

Pei formalism has been designed to reason and develop parallel programs in the context of data parallelism. In this paper, we focus on the use of Pei to transform a program involving dense matrices into a new program involving sparse matrices, using the example of the matrix-vector product.