Citrate lyase (EC 4.1.3.6) isolated from Rhodopseudomonas palustris was investigated with regard to its kinetic properties and its subunit composition. This enzyme was inactivated by citrate lyase deacetylase (EC 3.1.2.-) of Rhodopseudomonas gelatinosa. A corresponding cross-reaction was measured with partially purified deacetylase of R. palustris and citrate lyase of R. gelatinosa. The three d...

BACKGROUND Pectin is an abundant component in many fruit and vegetable wastes and could therefore be an excellent resource for biorefinery, but is currently underutilized. Fungal pectinases already play a crucial role for industrial purposes, such as for foodstuff processing. However, the regulation of pectinase gene expression is still poorly understood. For an optimal utilization of plant bio...

The gene encoding the pectin lyase (PNL; EC 4.2.2.10) of Bacillus subtilis has been cloned, sequenced, and characterized. A coding sequence for the PNL composed of 345 amino acids including a 24-amino-acid signal peptide was assigned. No sequence resembling a LexA binding site was found upstream of the structural gene. Furthermore, PNL activity of the gene product expressed in Escherichia coli ...

Pectins are a major component of the plant cell wall and comprise one of the two major coextensive networks in which cellulose microfibrils are embedded (Carpita and Gibeaut, 1993). Pectic polysaccharides exist in the cell wall as either “smooth” regions of a linear copolymer of a-(1– 4)-linked GalUA or “hairy” regions that have attached a-(1–2)-linked rhamnosyl residues that may be substituted...

The inducible alginate lyase of Klebsiella ptieitmoniue catalyses the degradation of guluronate-rich regions of the polysaccharide alginate [ I ] . The alginate lyase, which is chromosomally encoded [ 21, is largely secreted into the medium (31. although intracellular forms of the enzyme have been reported [4, 51. Recently, we have reported the cloning and heterologous expression in strains of ...

Because of its key role in secondary phenylpropanoid metabolism, Phe ammonia-lyase is one of the most extensively studied plant enzymes. To provide a basis for detailed structure-function studies, the enzyme from parsley (Petroselinum crispum) was crystallized, and the structure was elucidated at 1.7-A resolution. It contains the unusual electrophilic 4-methylidene-imidazole-5-one group, which ...

During the brine fermentation of cucumbers for pickles, Bell et al. (1950) have reported finding a fungal-type polygalacturonase-like enzyme which splits the polymer linkage of pectic substances. They definitely established this enzyme with the softening type of spoilage of salt-stock pickles brined under commercial conditions. With the identity of the softening agent known, an inquiry into its...