We investigated the effects of single and combined exposures to two ubiquitous environmental carcinogens, polycyclic aromatic hydrocarbons and UVA radiation, in Big Blue mouse embryonic fibroblasts. We quantified the cytotoxicity, DNA adduct formation, and induction of mutations in the cII transgene in cells treated with a single agent or combinations of agents in both direct and reverse order....

In this paper, we propose the use of partial differential equation (PDE) based novel PDE transforms for determining jump discontinuity locations in piecewise smooth data containting potential isolated jump discontinuities. The PDE transform method involves evolving coupled PDEs under different time scales to extract edge information. Multiscale edge detection is done by varying the parameters a...

Depolarized dynamic light scattering (DLS), dielectric relaxation (DS), and deuterium NMR studies of fragile van der Waals glass forming liquids phenylphthalein-dimethylether (PDE) and cresolphthalein-dimethylether (KDE) are presented. In PDE a new dielectric loss process was found, which can be attributed to the 180 degrees flip of the phenyl rings. The previous finding that the distribution o...

In their role as second messengers, cyclic nucleotides such as cAMP have a variety of intracellular effects. These complex tasks demand a highly organized orchestration of spatially and temporally confined cAMP action which should be best achieved by compartmentalization of the latter. A great body of evidence suggests that cAMP compartments may be established and maintained by cAMP degrading e...

Calmodulin-dependent phosphodiesterase (CaM-PDE) is selectively expressed in specific neuronal populations in adult rat brain. In cerebellar cortex, it is expressed at high levels in Purkinje cells (soma and dendrites). Climbing fiber ablation by intraperitoneal injections of 3-acetylpyridine resulted in a selective depression of cerebellar CaM-PDE expression using Western immunoblot procedures...

The promyelocytic HL60 cell can be differentiated with dimethyl sulphoxide or dibutyryl cyclic AMP leading to the appearance of fMetLeuPhe receptors on the cell surface. G-protein-stimulated polyphosphoinositide phosphodiesterase (PPI-pde) activity was assessed in membranes prepared from both differentiated and non-differentiated HL60 cells. Both the extent of the response and the rank order of...

Incubation of intact rat fat cells with maximally effective concentrations of insulin (1 nM, 12 min) or isoprenaline (300 nM, 3 min) increased particulate cGMP- and cilostamide-inhibited, low-Km cAMP phosphodiesterase (cAMP-PDE) activity by about 50% and 100%, respectively. In 32P-labeled cells, these agents induced serine 32P-phosphorylation of a 135-kDa particulate protein and, to a variable ...

CASE A 45-year-old man has a 10-year history of migraine without aura occurring about once a week relieved by an oral triptan. When he sought treatment for erectile dysfunction from a urologist, the side effect of triggering migraine was brought up. Now the patient and the urologist want my opinion about whether the patient should try an oral phosphodiesterase-5 inhibitor (PDE-5) and, if so, do...

Because phosphodiesterase (PDE) expression and activity are controlled by cAMP, we investigated whether activating mutations of Gs alpha gene that occur in human GH-secreting adenomas are associated with increased PDE activity. We studied 10 adenomas with wild-type Gs alpha (gsp-) and 8 with mutant Gs alpha (gsp+). Although, in the absence of PDE inhibitors, intracellular cAMP levels were simil...

Background: The increasing use of nuclear radia on for human welfare necessitates the search for new, safe, cost effec ve radio protectors not only for the personnel’s charged with the responsibility of working or tes ng with radia ons in laboratories, but also for general public. With this view the present study has been undertaken to determine the deleterious effects of sub lethal gamma radia...