Polymerase chain reaction (PCR)-based assays targeting the small-subunit rRNA were developed and evaluated, allowing for the simultaneous diagnosis of Plasmodium falciparum and Plasmodium vivax DNA in human blood samples. The PCR methods and quantitative buffy coat (QBC) were compared in 402 patients. The heminested PCR method showed a sensitivity of 97.4%, which was superior to the sensitivity...

The real-time polymerase chain reaction (PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the DNA amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect amplification products. Appropriate data analysis and/or use of apposite chemistries ...

Advances in biotechnology has been the subject of praise for a decade now, this is due to techniques such as gene expression which contributed immensely success genetic engineering, medical advancement, vaccine production and agriculture. Gene become very important tool overall improvement quality life. This paper tries look into development last forty years highlight how technological advancem...

Cost-effective/sensitive/selective/reliable detectors for firstresponders Point-of-care/clinical applications for pathogen/virus/threat detection and identification Technological challenges for rapid/early/specific sensitive detection Role of nanotechnology and system miniaturization; MEMS and bioMEMS Use of biochip technology; microand nanofluidics Bioinformatics for biodefense Reducing false ...

Cost-effective/sensitive/selective/reliable detectors for firstresponders Point-of-care/clinical applications for pathogen/virus/threat detection and identification Technological challenges for rapid/early/specific sensitive detection Role of nanotechnology and system miniaturization; MEMS and bioMEMS Use of biochip technology; microand nanofluidics Bioinformatics for biodefense Reducing false ...

Agarose emulsion droplet microfluidic technology for single copy emulsion PCR (ePCR) is a suitable technique for the detection of single copy DNA molecules. It improves the traditional ePCR by employing agarose with low melting and low gelling temperatures, which is coupled with PCR forward primers using Schiff-base reaction. Highly uniform monodisperse nanoliter agarose droplets each containin...

The use of genetically modified organisms (GMOs) as food and in food products is becoming more and more widespread. Polymerase chain reaction (PCR) technology is extensively used for the detection of GMOs in food products in order to verify compliance with labeling requirements. In this paper, we present a novel close-loop ferrofluid-driven PCR microchip for rapid amplification of GMOs. The mic...

BACKGROUND Pyrosequencing technology has the potential to rapidly sequence HIV-1 viral quasispecies without requiring the traditional approach of cloning. In this study, we investigated the utility of ultra-deep pyrosequencing to characterize genetic diversity of the HIV-1 gag quasispecies and assessed the possible contribution of pyrosequencing technology in studying HIV-1 biology and evolutio...

Biological warfare agents are the most problematic of the weapons of mass destruction and terror. Both civilian and military sources predict that over the next decade the threat from proliferation of these agents will increase significantly. In this review we summarize the state of the art in detection and identification of biological threat agents based on PCR technology with emphasis on the n...

The application of microfluidic devices for DNA amplification has recently been extensively studied. Here, we review the important development of microfluidic polymerase chain reaction (PCR) devices and discuss the underlying physical principles for the optimal design and operation of the device. In particular, we focus on continuous-flow microfluidic PCR on-chip, which can be readily implement...