Linkage studies have been carried out in 20 families segregating for Duchenne muscular dystrophy and eight prenatal diagnoses performed, including six first trimester diagnoses and one twin pregnancy. The results of the restriction fragment length polymorphism (RFLP) analysis suggest that not all the possible RFLPs need to be used and a strategy for carrier detection studies is proposed.

• Define genetic linkage and recombinant frequency • State how genetic distance may be estimated • State how restriction enzymes can be used for isolating genes • Define Restriction Fragment Length Polymorphisms (RFPLs) • Identify the main applications of RFLPs in gene mapping and carrier detection • State the principles used in identifying a specific gene in the genome by hybridisation with a ...

Restriction fragment length polymorphisms (RFLPs) are useful in linkage and clinical association studies of human diseases. In this report, we characterize the molecular basis and frequencies of two new RFLPs, AvaII and BalI, two previously reported RFLPs, HincII and PvuII, and one new sequence polymorphism in the human apolipoprotein B gene. For the AvaII RFLP, the two alleles yield either a 1...

Ribotyping, a molecular method based on the analysis of restriction fragment length polymorphisms (RFLPs) of ribosomal RNA genes, has been widely adopted to distinguish a variety of bacteria (1-5). For the past five years ATCC has been using the RiboPrinter® Microbial Characterization system (Qualicon Inc., Wilmington, DE) as a primary method for characterizing strains during the quality contro...