نتایج جستجو برای: pcr multiplex
تعداد نتایج: 186945 فیلتر نتایج به سال:
A multiplex PCR designed to differentiate Mycobacterium tuberculosis complex organisms from M. avium and M. intracellulare was used to test 105 isolates identified by DNA probe methods as M. avium, M. intracellulare, or M. avium complex type X. The multiple PCR correctly identified 33 of 34 isolates identified by commercial probe methods as M. avium and all 51 isolates identified as M. intracel...
A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was developed. Platinum® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction in approximately 2.5 hours . In order to shorten the process with reliable results, several DNA polymerases were tested with the multiplex. Phusion® Flash High Fidelity and Platinum® Taq DNA polymerases were inv...
Introduction: Contamination with high-risk human papillomavirus (HPV) is one of the most important risk factors for developing cervix cancer. Since there is no possibility of detecting the virus and its subtypes by using current biochemical and serological methods and cell culture, the molecular methods such as multiplex PCR have particular importance in accurate, early and definite diagnosis o...
Bacteriological culture was compared with multiplex and fluorogenic (TaqMan) polymerase chain reaction (PCR) assays for the detection of attachment invasion locus (ail)-bearing Yersinia enterocolitica in market weight swine, chitterlings, and ground pork. The TaqMan assay detected 1 pg of purified Y. enterocolitica DNA, whereas conventional gel-based PCR detected I ng of the same. The presence ...
This study explored epidemiological trends in trichomoniasis in Daegu, South Korea. Wet mount microscopy, PCR, and multiplex PCR were used to test for Trichomonas vaginalis in vaginal swab samples obtained from 621 women visiting 2 clinics in Daegu. Of the 621 women tested, microscopy detected T. vaginalis in 4 (0.6%) patients, PCR detected T. vaginalis in 19 (3.0%) patients, and multiplex PCR ...
BACKGROUND Due to an increasing number of norovirus infections in the last years rapid, specific, and sensitive diagnostic tools are needed. Reverse transcriptase-polymerase chain reactions (RT-PCR) have become the methods of choice. To minimize the working time and the risk of carryover contamination during the multi-step procedure of PCR the multiplex real-time RT-PCR for the simultaneous det...
clostridium perfringens (c. perfringens) is related to gram-positive and anaerobic bacteria. this rod-shaped bacterium produces spores and can cause a wide spectrum of diseases in humans and animals. every of five different bacterium types can lead to different diseases. most of distinct bacterial recognition methods are not completely effective and practical in all condition. the objective of ...
سابقه و هدف: امروزه مصرف گسترده بتالاکتامهای وسیع الطیف موجب افزایش مقاومت به بتالاکتامها در سراسر دنیا شده است. هدف این تحقیق، بررسی و شناسایی ژنهای blatem، blashv،blaoxa و آمینوگلیکوزیدی aada در ایزولههای بالینی اشریشیاکلی به روش pcr multiplex بود. روش بررسی: در این مطالعه توصیفی، تعداد 150 نمونه مدفوع اسهالی از مراکز درمانی مختلف شهر تهران که به صورت تصادفی انتخاب شده بودند، جمعآوری و ...
سابقه و هدف: انتروکوک ها، پاتوژن های مهم و شایع در عفونت های بیمارستانی هستند. ازمیان گونه های مختلف انتروکوک ها، انتروکوکوس فکالیس و انتروکوکوس فسیوم، پاتوژن های اصلی محسوب می شوند. سویه های مقاوم به ونکومایسین (vre: vancomycin resistant enterococci) در بیماران سرپایی و بستری به وفور در سراسر جهان مشاهده می شوند. در این مطالعه، خصوصیات فنوتیپی و ژنوتیپی سویه های ایزوله شدهvre از بیماران بستری ...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید