A Lactobacillus group-specific PCR primer, S-G-Lab-0677-a-A-17, was developed to selectively amplify 16S ribosomal DNA (rDNA) from lactobacilli and related lactic acid bacteria, including members of the genera Leuconostoc, Pediococcus, and WEISSELLA: Amplicons generated by PCR from a variety of gastrointestinal (GI) tract samples, including those originating from feces and cecum, resulted predo...

BACKGROUND Candida species are usually opportunistic organisms that cause acute to chronic infections when conditions in the host are favorable. Accurate identification of Candida species is an essential pre-requisite for improved therapeutic strategy. Identification of Candida species by conventional methods is time-consuming with low sensitivity, yet molecular approaches have provided an alte...

Background and Aim: Oral infections are usual between people of any age and can activate systemic inflammation. The microbiota consists of a variety of microorganisms that play an important role in metabolism, immune function, and homeostasis. Oral microbiota in human atherosclerotic plaques has been identified by various techniques. Therefore, the focus of study was to determine the correlatio...

Rice field soil with a nonsaturated water content induced CH4 consumption activity when it was supplemented with 5% CH4. After a lag phase of 3 days, CH4 was consumed rapidly until the concentration was less than 1.8 parts per million by volume (ppmv). However, the soil was not able to maintain the oxidation activity at near-atmospheric CH4 mixing ratios (i.e., 5 ppmv). The soil microbial commu...

For many ecological studies of cyanobacteria, it is essential that closely related species or strains can be discriminated. Since this is often not possible by using morphological features, cyanobacteria are frequently studied by using DNA-based methods. A powerful method for analysis of the diversity and dynamics of microbial populations and for checking the purity and affiliation of cultivate...

Effluent from a fish tank with Nile tilapia (Oreochromis niloticus) was treated in system of two parallel fixed-bed anaerobic reactors followed by suspended stirred, anoxic reactor for solids, organic matter and nitrogen removal. Microbial community structure compared between the an enriched anammox culture originated sludge using PCR (Polymerase Chain Reaction) DGGE (Denaturing Gradient Gel El...

The combined action of physical protection and chemical stabilisation is the main factor regulating persistence soil organic matter (SOM). In particular, refers to compartmentalization SOM microbial biomass within aggregates. Thus, we have investigated role (macro-, meso- micro-) aggregates in extracellular intracellular DNA (eDNA vs iDNA) from oxidative stress by treating with Low Temperature ...