Polymorphism. Two polymorphic microsatellite loci, BFRO010 and BFRO011, were identified in genomic DNA of grayling (Thymallus thymallus). Source and Description. The template DNA was isolated from a library of size-selected (200 to 800 bp) Sau3AI genomic restriction fragments from grayling. The DNA fragments were cloned into pBluescript II SK+ (Stratagene, La Jolla, CA) vector, previously restr...

It is shown that for each integer k > 3, there exists a set Sk of positive integers containing no arithmetic progression of k terms, such that 2„6Si \/n > (1 e)k log A:, with a finite number of exceptional k for each real e > 0. This result is shown to be superior to that attainable with other sets in the literature, in particular Rankin's sets &(k), which have the highest known asymptotic dens...

Fed-batch culture strategy is often used for increasing production of heterologous recombinant proteins in Escherichia coli. This study was initiated to investigate the effects of dissolved oxygen concentration (DOC), complex nitrogen sources and pH control agents on cell growth and intracellular expression of streptokinase (SK) in recombinant E. coli BL21(DE3). Increase in DOC set point from 3...

Sphingolipid-metabolizing enzymes control the dynamic balance of the cellular levels of bioactive lipids, including the proapoptotic compound ceramide and the proliferative compound sphingosine 1-phosphate. Accumulating evidence indicates that sphingosine kinase (SK) plays a pivotal role in regulating tumor growth and that SK can act as an oncogene. Despite the importance of SK for cell prolife...

Sphingolipid-metabolizing enzymes control the dynamic balance of the cellular levels of bioactive lipids, including the proapoptotic compound ceramide and the proliferative compound sphingosine 1-phosphate. Accumulating evidence indicates that sphingosine kinase (SK) plays a pivotal role in regulating tumor growth and that SK can act as an oncogene. Despite the importance of SK for cell prolife...

human granulocyte colony stimulating factor (hg-csf) induces proliferation and differentiation of granulocyte progenitor cells. this glycoprotein is currently being used for treatment of neutropenia, in patients who have undergone bone marrow transplantation. so far, different researchers have tried to enhance hg-csf biological activity and stability. in this study, polymerase chain reaction (p...

In this paper we describe some features of the parameter planes for the families of rational maps given by Fλ(z) = z n + λ/zn where n ≥ 3, λ ∈ C. We assume n ≥ 3 since, in this case, there is a McMullen domain surrounding the origin in the λ-plane. This is a region where the corresponding Julia sets are Cantor sets of concentric simple closed curves. We prove here that the McMullen domain in th...