PURPOSE Mammalian extraocular muscles (EOMs) are both physiologically and biochemically unique when compared with nonocular skeletal muscles. Recent studies have demonstrated a process of continuous myonuclear addition in normal uninjured myofibers in adult EOMs of rabbits and mice. The current study was conducted to determine whether this process of myonuclear addition is a universal phenomeno...

Despite the widespread consumption of nonsteroidal anti-inflammatory drugs (NSAIDs), the influence of these drugs on muscle satellite cells is not fully understood. The aim of the present study was to investigate the effect of a local NSAID infusion on satellite cells after unaccustomed eccentric exercise in vivo in human skeletal muscle. Eight young healthy males performed 200 maximal eccentri...

From studies in the mouse and from the clinical and molecular analysis of patients with type 1 Waardenburg syndrome, particular members of the PAX gene family are suspected factors in the aetiology of human neural tube defects (NTD). To investigate the role of PAX1, PAX3, PAX7, and PAX9, allelic association studies were performed in 79 sporadic and 38 familial NTD patients from the Dutch popula...

PURPOSE This study determined the molecular characteristics and clinical significance of amplification of the 13q31 chromosomal region in alveolar rhabdomyosarcoma (ARMS), an aggressive pediatric cancer with frequent PAX3-FOXO1 and PAX7-FOXO1 gene fusions. EXPERIMENTAL DESIGN The 13q31 amplicon was localized in an initial panel of ARMS cases using oligonucleotide arrays. A fluorescence in sit...

Ubiquitin-specific protease 8 (USP8), a member of the ubiquitin-specific (USP) family, was originally identified as playing role in regulation growth and cell cycle. However, its functional myogenesis is unknown. In this study, we investigated USP8 proliferation differentiation sheep skeletal muscle satellite cells. The results showed that expression level significantly increased on days 2 3 fo...

PURPOSE The goal of this study was to determine whether the medial rectus muscles of patients with a history of medial rectus underaction or overaction show alterations in the process of satellite cell activation when compared with normal age-matched control muscles. METHODS Medial rectus muscles were obtained with consent from adult patients undergoing surgical resection due to medial rectus...

A popular theory in the stem cell field is that 'regeneration recapitulates development', or that adult stem cells function similarly to embryonic ones. In a recent Nature article, Lepper et al. questioned this approach, highlighting the differences in requirements for Pax7 during myogenesis for embryonic, juvenile and adult muscle.