Background/Aim: Recent studies suggested a risk of gastric adverse reaction on the concomitant use of antiplatelet drugs with NSAIDs. The present study was performed to examine the adverse effects of anti-platelet drug clopidogrel, a P2Y12 receptor antagonist, on gastric antral lesions induced by conventional NSAIDs in refed

In the search for P2-receptors modulating the stimulation-evoked entry of calcium at processes of PC12 cells differentiated in the presence of nerve growth factor and neurotrophin-3, electrically evoked increases in free calcium were assessed by fura-2 microfluorimetry. Omission of calcium and addition of cadmium (100 microM) or the N-type calcium channel blocker omega-conotoxin GVIA (0.5 micro...

Human platelets possess 3 purinergic receptors (P2Y12, P2Y 1 , and P2X 1 ), which collectively orchestrate key steps leading to platelet activation and aggregation (Figure). Until now, the selective blockade of the platelet P2Y 12 ADP receptor, combined with the inhibition of thromboxane production by aspirin, has remained the backbone of pharmacotherapy for patients presenting with acute coron...

Adenosine diphosphate (ADP), an important platelet agonist, acts through 2 Gprotein–coupled receptors (GPCRs), P2Y1 and P2Y12, which signal through Gq and Gi, respectively. There is increasing evidence for cross-talk between signaling pathways downstream of GPCRs and here we demonstrate cross-talk between these 2 ADP receptors in human platelets. We show that P2Y12 contributes to platelet signa...

Background Animal models of allergic asthma indicate that intravascular platelet activation is essentail for the development of allergen induced chronic airway inflammation. P2Y12, the third CysLT receptor, is expressed on platelets and has been an important pathophysiological role in LTE4 mediated pulmonary inflammation. We investigated platelet activation status in asthmatic patients compared...

P2Y12, one of the two platelet receptors for adenosine diphosphate (ADP), plays a central role in platelet function. Defects of P2Y12 should be suspected when ADP, even at high concentrations (≥10 µM), is unable to induce full, irreversible platelet aggregation. Patients with congenital P2Y12 defects display a mild-to-moderate bleeding diathesis of variable severity, characterised by mucocutane...

The P2Y receptor family is a class of G protein-coupled receptors activated primarily by adenosine triphosphate (ATP), diphosphate (ADP), uridine (UTP) and (UDP). P2Y12 expressed on platelets which mediates platelet aggregation morphological changes. At the same time, during process vascular remodeling atherosclerosis, ADP can also promote migration proliferation smooth muscle endothelial cells...

We have previously shown that ADP-induced thromboxane generation in platelets requires signalling events from the G(q)-coupled P2Y1 receptor (platelet ADP receptor coupled to stimulation of phospholipase C) and the G(i)-coupled P2Y12 receptor (platelet ADP receptor coupled to inhibition of adenylate cyclase) in addition to outside-in signalling. While it is also known that extracellular calcium...