AIM Annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. This study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in Gwagwalada market, Abuja, and recommend appropriate control measure. MATERIALS AND METHODS The study was conducted in Gwagwalada metropolis, Gwagwalada Area Council of the Federal Capital ...

Aspergillus niger is a common clinical isolate. Multiple species comprise the Aspergillus section Nigri and are separable using sequence data. The antifungal susceptibility of these cryptic species is not known. We determined the azole MICs of 50 black aspergilli, 45 from clinical specimens, using modified EUCAST (mEUCAST) and Etest methods. Phylogenetic trees were prepared using the internal t...

Transgenic filamentous fungi of the species Aspergillus niger, A. nidulans and A. awamori expressing and secreting Erwinia carotovora (Eca) subsp. atroseptica pectate lyase 3 (PL3) were generated. Correct processing of the pre-enzyme was achieved using the A. niger pectin lyase A (PEL A) signal peptide. With the prepro-peptide of A. niger polygalacturonase II, secreted enzymes still possessed t...

The Niger Delta is dying. Petrobusiness activities have caused severe environmental damage and climate change in its communities thus leading to massive destruction of farmlands, wild and marine lives. This has not been without dire social consequences on local communities of the Niger Delta which are doubly impoverished with attendant increase in abuse occasioned by struggle for survival. This...

Antimicrobial activity of 25, 50, 75 and 100% alcohol extract of Piper nigrum (Black Pepper), Psidium guajava (Guava), Amomum cardamom (Cardamom) and Cynogen dactylon (Bermuda grass) has been evaluated against Pseudomonas lundensis, Bacillus cereus, Aspergillus niger and Aspergillus flavus. Psidium guajava extract showed excellent antimicrobial activity against all the test organisms. In 25% co...

Citric acid is regarded as a metabolite of energy metabolism, of which the concentration will rise to appreciable amounts only under conditions of substantive metabolic imbalances. Citric acid fermentation conditions were established during the 1930s and 1940s, when the effects of various medium components were evaluated. The biochemical mechanism by which Aspergillus niger accumulates citric a...

In the biosynthesis of tropane alkaloids, two tropinone reductases (TRs) catalyze reduction of tropinone to different stereoisomers, tropine and pseudotropine. Two TRs from Hyoscyamus niger have 64% of identical amino acids and hence a common evolutionary origin. In this study, genomic clones of TRs were isolated from H. niger. Their sequence comparison showed that although they have the same e...

The effect of two fungicides, Caocobre and Ridomil on rhizosphere phenomenon of Cocoa (Theobroma cacao L) seedlings has been studied at different stages of the development of the plant growing in pots. Fungal population in the rhizophere soil was generally higher than in the non-rhizosphere soil. The total number of fungal population in both the rhizosphere and non-rhizosphere was highest on th...

In a recent issue of PNAS, Scheinfeldt et al. (1) maintained that, although Fulani mtDNA is consistent with a West African origin, the linguistic and nonrecombinant portion of the Y chromosome (NRY) supports a Middle Eastern origin for this population. Although this is their opinion, the linguistic and genetic evidence fails to support this conclusion. The Fulani speak a Niger-Congo language. T...

BACKGROUND The amino acid derivative 3,4-dihydroxy L-phenylalanine (L-dopa) is gaining interest as a drug of choice for Parkinson's disease. Aspergillus oryzae is commonly used for L-dopa production; however, a slower growth rate and relatively lower tyrosinase activity of mycelia have led to an increasing interest in exploiting alternative fungal cultures. In the present investigation, we repo...