This present study was undertaken to investigate the ultrasonic delignification of switchgrass (Panicum virgatum L.) and effects irradiation on molecular microstructure switchgrass. We investigated this question using response surface methodology (RSM) featuring a four-factor, three-level Box–Behnken experimental design with acoustic power (120, 180, 240 W), solid–solvent ratio (1/25, 1/20, 1/1...

In kraft pulping, wood polysaccharides are subjected to the end-wise degradation (i.e., the peeling reaction) under alkaline conditions with the simultaneous formation of a large number of water/alkali-soluble hydroxy monoand dicarboxylic acids together with formic and acetic acids [1-3]. The versatile analysis of organic material in black liquor is of a great importance, for example, for monit...

The effects of cellulose crystallinity, hemicellulose, and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides were investigated. A air-dried biomass was ground by ball-milling, and the powder was separated into four fractions by passage through a series of sieves with mesh sizes 250-355 microm, 150-250 microm, 63-150 microm, and <63 microm. Each fraction was hydrolyzed...

CRYOSTAT MICROTOME SECTIONS OF BIRCH WOOD DEGRADED BY WHITE ROT FUNGI WERE EXAMINED BY LIGHT MICROSCOPY AFTER TREATMENT WITH TWO STAINS: astra-blue, which stains cellulose blue only in the absence of lignin, and safranin, which stains lignin regardless of whether cellulose is present. The method provided a simple and reliable screening procedure that distinguishes between fungi that cause decay...

Paper pulp delignification using laccase and natural mediators Susana Camarero a, David Ibarra a, Ángel T. Martı́nez a,∗, Javier Romero b, Ana Gutiérrez c, José C. del Rı́o c a Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain b Centro de Investigación y Tecnologı́a, ENCE, Carretera de Campañó, Ribeiro Vao, E-36157 Pontevedra, Spain c Instituto de Recursos Natur...

The potential of the lipoxygenase from Gaeumannomyces graminis to remove lipophilic extractives from eucalypt and flax pulps was investigated. Pulp treatments were performed with the lipoxygenase both in the presence and absence of linoleic acid, and were followed by a peroxide bleaching stage. The main lipophilic extractives from eucalypt pulp such as conjugated and free sterols decreased up t...

BACKGROUND Ligninolytic peroxidases are divided into three families: manganese peroxidases (MnPs), lignin peroxidases (LiPs), and versatile peroxidases (VPs). The latter two are able to degrade intact lignins, as shown using nonphenolic lignin model compounds, with VP oxidizing the widest range of recalcitrant substrates. One of the main limiting issues for the use of these two enzymes in ligno...