ovine pulmonary adenocarcinoma (opa) was studied in the lungs of 15 naturally-affected sheep (9 with classical and 6 atypical lesions) by transmission electron microscopy. two lung samples from normal sheep were used to develop the ultrastructural criteria. pathologic lesions consisted of focal-multifocal to coalescent nodules or masses in the cranioventral or diaphragmatic lobes. ultrastructur...

theileria species are common in tropical and subtropical regions and cause great economical losses inruminants. two species, t. lestoquardi and t. ovis, are suspected to cause ovine theileriosis in iran. theepidemiological aspects of ovine theileriosis in iran are poorly understood and further investigations bysensitive and precise techniques are required. in a previous study, a sensitive and s...

Mouse placental lactogen was purified 1840-fold from BALB/c placentae from days 14-18 of gestation with an overall yield of 29%. The purification procedure included alkaline homogenization and extraction, ammonium sulfate precipitation, hydrophobic interaction chromatography on Phenyl-Sepharose, ion-exchange chromatography on CM- and DEAE-cellulose, and gel exclusion chromatography on Sephadex ...

Natural killer (NK) cells are well recognized as playing a key role in innate immune defence through cytokine production and cytotoxic activity; additionally recent studies have identified several novel NK cell functions. The ability to study NK cells in the sheep has been restricted due to a lack of specific reagents. We report the generation of a monoclonal antibody specific for ovine NKp46, ...

BACKGROUND Endothelial cell co-culture assays are differentiation assays which simulate the formation of capillary-like tubules with the aid of a supportive cell layer. Different cell types have been employed as a supportive cell layer, including human pulmonary artery smooth muscle cells (PASMCs) and human mammary fibroblasts. However, these sources of human tissue-derived cells are limited, a...

BACKGROUND Loss of meniscal tissue is correlated with early osteoarthritis but few data exist regarding detailed biomechanical properties (e.g. viscoelastic behavior) of menisci in different species commonly used as animal models. The purpose of the current study was to biomechanically characterize bovine, ovine, and porcine menisci (each n = 6, midpart of the medial meniscus) and compare their...

概要 In vivoで 肺血管外水分量(pulmonary extravascular water volume, PEV)を測 定す るこ とは,1954年Chinard & Ennsに よ り示唆 されて以来,基 礎的検討がか さね られ臨床への応用が可 能 となつた.本 論文 は,Ramseyら の方法 に準 じて後天性 心疾患に おけ るPEVを 測定 し,肺 循環諸 量,臨 床経過 お よび血液 ガス値 との関連について検 討 した ものである。測定時 の肺血管内圧 とPEV とは,粗 な正相 関(r=0.48,P<0.02)を 示 すにす ぎず,む しろPEVは 左心不全の既往 と関連 が あ る とい う結果 をえた.す なわ ち測定時 の肺 血管 内圧は同 じレベ ルにあ りなが ら,左 心不 全既往のあ る例は,既 往 のない例 に比ベ てPEVは 著 明な増加を示...

Accession Number. U15731. Species. Bos taurus. Origin of Clone. Hybridizing with a 220-bp cDNA of the somatotropin receptor (Hauser et al., 1990) 10 clones were identified from a bovine genomic library (EMBL3 SP6pT7 cloning vector; Clontech Laboratories, Palo Alto, CA). A 2.9-kb XhoIIAatII fragment containing the promoter and exon I was subcloned into pGEM-7Zf (Promega, Madison, WI), and both s...

Recent findings from this laboratory suggest that the biological actions of placental lactogen (PL) in mammalian fetal tissues are mediated through binding of the hormone to a distinct and unique PL receptor. We have now purified this receptor from fetal and maternal sheep liver, characterized its binding to PL, growth hormone (GH), and prolactin (PRL), and determined its molecular weight by SD...

Staphylococcus aureus is an important pathogen of many species, including sheep, and impacts on both human and animal health, animal welfare, and farm productivity. Here we present the widest global diversity study of ovine-associated S. aureus to date. We analysed 97 S. aureus isolates from sheep and sheep products from the UK, Turkey, France, Norway, Australia, Canada and the USA using multil...