Figure 1. Diagram of a split-marker gene replacement strategy using fusion-PCR (A) FPCR step 1 amplification of flanking regions and partial marker segments. Dotted tails indicate the overlap sequences on selected primers which allow fusion in step 2. (B) FPCR step 2 flanking regions are fused to the marker segments at the overlap sights, forming two constructs. The fusion products are then amp...

Single-nucleotide primer extension (SNuPE) is an emerging tool for parallel detection of DNA sequences of different target microorganisms. The specificity and sensitivity of the SNuPE method were assessed by performing single and multiplex reactions using defined template mixtures of 16S rRNA gene PCR products obtained from pure bacterial cultures. The mismatch discrimination potential of prime...

Recently PCR is being used more frequently as a diagnostic method to detect M. pneumoniae. We used primer pairs reported by Van Kuppeveld,13 Leng,5 Lunerberg,7 an Bernet' targeting 16s rRNA, PI protein, tuf genes. and a short DNA equence (MP5) to evaluate the sensitivity among different PCRs. Reoptimization experiments showed that tuf PCR had the highest sensitivity amongst these four PCRs, det...

Traditional clustering algorithms, such as k-means, output a clustering that is disjoint and exhaustive, that is, every single data point is assigned to exactly one cluster. However, in real datasets, clusters can overlap and there are often outliers that do not belong to any cluster. This is a well recognized problem that has received much attention in the past, and several algorithms, such as...

Name disambiguation has become one of the main themes in the Semantic Web agenda. The semantic web is an extension of the current Web in which information is not only given well-defined meaning, but also has many purposes that contain the ambiguous naturally or a lot of thing came with the overlap, mainly deals with the persons name. Therefore, we develop an approach to extract keywords from we...