We present a case in which kappa free light chains caused difficulty in interpreting classical urinary immunoelectrophoresis, but immunofixation electrophoresis (IFE) demonstrated the presence of a lambda-Bence Jones protein. Analysis of the urine by Ouchterlony double diffusion and IFE after gel-filtration chromatography showed that the difficulty was caused by the presence of large amounts of...

Antibodies to broad bean polyphenoloxidase (PPO) were used to detect and demonstrate that the PPOs found in several different plants have many similarities in common. Crude extracts from leaves of broad bean, bush bean, lettuce, mung bean, pea, soybean, spinach, tobacco, and tomato contained enzyme which cross-reacted with polyclonal anti-PPO in Ouchterlony double diffusion analysis. The result...

Until 1950 the differentiation of the KochWeeks bacillus and Haemophilus influenzae had not been well defined and their identity as separate species or single species has been controversial. However the results reported by Pittman and Davis (1950) showed that the Koch-Weeks bacillus, Haemophilus aegyptius, is a distinct species that can be separated from H. influenzae by serological means and, ...

SUMMARY These studies indicate that granulosa cell and granulosa theca cell cancers of the ovary contain two common tumor associated antigenic components that are apparently absent from normal ovaries and other histologically similar normal organs. The tumor antigens were immunologically distin guishable from the tumor antigens ofepithelial ovarian cyst adenocarcinomas but were indistinguishabl...

The rabbit anterior chamber was used for implantation of specifically sensitized allogeneic lymph node tissue. Initial vascularizaton was followed by a primary uveitis corresponding to a local graft-versus-host reaction (GVHR) which resolved after 4 to 6 days. Intracorneal injection of specific antigen in quiescent eyes bearing viable lymph node allografts was followed by an intense inflammator...

The chick kidney mitochondrial iron-sulphur protein (ferredoxin), a component of the NADPH-cytochrome P-450 reductase functional in the 1 a-hydroxylation of 25-hydroxycholecalciferol, was purified to homogeneity by chromatography on DEAE-cellulose, gel filtration on Sephadex G-100 and preparative electrophoresis on polyacrylamide gel. A novel NADPH-cytochrome c reductase assay utilizing crude r...

The total cytosol activity of acetyl-CoA carboxylase (acetyl-CoA:CO(2) ligase (ADP), EC 6.4.1.2) in the liver is known to be 6- to 10-fold higher in genetically obese hyperglycemic mice (C57BL/6J-ob) than in nonobese mice. The results of immunochemical titrations, Ouchterlony double-diffusion analysis, and kinetic and heat inactivation studies indicated that this rise in the level of carboxylas...

Prostate specific antigen (PSA, also known as p30), a glycoprotein produced by the prostatic gland and secreted into seminal plasma, is a marker used for demonstrating the presence of seminal fluid. Methods for the detection of PSA include Ouchterlony double diffusion, crossover electrophoresis, rocket immuno-electrophoresis, radial immunodiffusion, and ELISA. The extremely sensitive ELISA tech...

Elevated concentrations of fibronectin were found in plasma of rats under different acute phase conditions. Untreated animals showed a plasma fibronectin concentration of 150 +/- 50 mg/l, which increased to 412 +/- 59 mg/l 24 h after subcutaneous injection of turpentine. The time course of the changes in plasma fibronectin concentration showed a peak at 24 h and a decline to normal concentratio...

Euglena gracilis (1224-5/9) contains phosphoenolpyruvate carboxykinase when grown autotrophic with CO(2) in the light. Its yield is higher when an additional carbon source like glucose has been added. The enzyme is lacking in cells provided with CO(2) alone and kept in the dark, whereas highest yields result if both glucose and CO(2) are provided together in the dark. The enzyme was purified by...