The conservation of genetic resources in cryocollections requires reliable protocols for the cryopreservation and regeneration preserved material. With Ulmus glabra, thawed buds by vitro organogenesis has suffered from low shoot growth high contamination rates. dehydration before improved rate ameliorated cultures initiated buds, although degree success varied depending on donor tree.

The study evaluated the aseptic establishment of Monstera acuminata Koch and deliciosa Liebm (Araceae) from leaves induction in vitro organogenesis M. K. stem discs young shoots. For this purpose, different disinfection protocols were applied to mature shoots, which leaf explants approximately 1 cm2 mm thickness extracted. established semi-solid media with hormone treatments during stage organo...

Background - MaterialsAndMethods N;Results N;Conclusion N;

The technique pioneered by D.A.T. New for the in vitro culture of early post-implantation rat embryos has been used to study nutritional mechanisms during early organogenesis. The results indicate that the principal route for amino acid supply to the 8.5- to 11.5-day embryo involves the endocytosis of proteins into cells of the visceral yolk sac endoderm, their digestion in lysosomes, and trans...

Laminins (LM) are extracellular matrix molecules that contribute to and are required for the formation of basement membranes. They participate in the modulation of epithelial/mesenchymal interactions and are implicated in organogenesis and maintenance of organ homeostasis. Among the LM molecules, the LM α5 chain (LMα5) is one of the most widely distributed LM in the developing and mature organi...

One novel solution to the shortage of human organs available for transplantation envisions growing new organs in situ via xenotransplantation of developing primordia from animal embryos. It has been shown that renal primordia (metanephroi) transplanted into animal hosts undergo organogenesis in situ, become vascularized by blood vessels of host origin, and exhibit excretory function. Metanephro...

Organ formation during embryogenesis is a complex process that involves various local cell-cell interactions at the molecular and mechanical levels. Despite this complexity, organogenesis can be modelled in vitro. In this article, we focus on two recent examples in which embryonic stem cells can self-organise into three-dimensional structures - the optic cup and the pituitary epithelium; and on...

Prostate organogenesis is a complex process that is primarily mediated by the presence of androgens and subsequent mesenchyme-epithelial interactions. The investigation of prostate development is partly driven by its potential relevance to prostate cancer, in particular the apparent re-awakening of key developmental programs that occur during tumorigenesis. However, our current knowledge of the...

Pre-gestational diabetes (either type 1 or type 2) has the potential to subject the developing fetus to abnormal maternal glucose levels resulting in problems with organogenesis, resulting in congenital anomalies or spontaneous abortion. Whereas in, Gestational Diabetes Mellitus (GDM) which manifests after organogenesis in the second part of pregnancy, therefore the major risk for the fetus is ...

GreenLab Model is a functional-structural plant growth model that combines both organogenesis (at each cycle, new organs are created with respect to genetic rules) and photosynthesis (organs are filled with the biomass produced by the leaves photosynthesis). Our new developments of the model concern the retroaction of photosynthesis on organogenesis. We present here the first step towards the t...