نتایج جستجو برای: optical microscopy
تعداد نتایج: 442649 فیلتر نتایج به سال:
Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure. Recent development of super-resolution fluorescence microscopy allows the location of molecules to be determined with nanometer-scale spatial resolution. However, correlative super-resolution fluorescence microscopy and...
Atomic force microscopy (AFM) has evolved from the originally morphological imaging technique to a powerful and multifunctional technique for manipulating and detecting the interactions between molecules at nanometer resolution. However, AFM cannot provide the precise information of synchronized molecular groups and has many shortcomings in the aspects of determining the mechanism of the intera...
An association between DNA fragmentation in sperm determined by the terminal deoxynucleotidyl transferase [TdT]-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) assay and the incidence of reproductive failure has been reported, either using flow cytometry or optical microscopy. However, the results obtained using each of these two approaches are different. Since there is a re...
ZnO and Cu doped[1] (CZO) thin films were prepared by radio frequency sputtering. The structural and optical properties of thin films were investigated using X-ray diffraction (XRD), atomic force microscopy (AFM), optical spectrophotometer, and photoluminescence (PL) techniques. ZnO thin films showed crystalline and micro-stress defects in the crystal lattice. Annealing of CZO thin films increa...
Zigzag ZnS thin films prepared by thermal evaporation method using glancing angle deposition (GLAD) technique. ZnS films with zigzag structure were produced at deposition angles of 0˚, 60˚ and 80˚ at room temperature on glass substrates. Surface morphology of the films w:as char:acterized by using field emission scanning electron microscopy (FESEM). The optical properties of the specimens were i...
Diffraction limits standard optical microscopy to a spatial resolution of about half the wavelength of light. We present novel microscopy techniques for imaging semiconductor materials and devices at better than diffraction-limited spatial resolution and observe previously inaccessible phenomena. We discuss the methods and results for Numerical Aperture Increasing Lens (NAIL) and Near-field Sca...
In this research, ZnO nanostructure hexagonal pyramid rods with high optical and structural quality were synthesized by the simple thermal chemical vapor deposition of Zn powder without a metal catalyst. Surface morphologies were characterized by scanning electron microscopy (SEM). XRD analyses demonstrated that ZnO hexagonal pyramid rods had a wurtzite structure with the orientation of (002). ...
We discuss our recent results as well as planned experiments for obtaining molecular resolution in optical microscopy. By combining single molecule spectroscopy and scanning probe microscopy we have demonstrated optical microscopy with a single-molecule light source. We also sketch a method for the use of an inhomogeneous static electric field for determining the position of a molecule in all t...
As a revolutionary observation tool in life science, biomedical, and material optical microscopy allows imaging of samples with high spatial resolution wide field view. However, conventional methods are limited to single cannot accomplish real-time image processing. The edge detection, enhancement phase visualization schemes have attracted great interest the rapid development analog computing. ...
This article reports the development of an optical imaging technique, confocal light absorption and scattering spectroscopic (CLASS) microscopy, capable of noninvasively determining the dimensions and other physical properties of single subcellular organelles. CLASS microscopy combines the principles of light-scattering spectroscopy (LSS) with confocal microscopy. LSS is an optical technique th...
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