Targeted delivery represents a promising approach for the development of safer and more effective therapeutics for oncology applications. Although macromolecules accumulate nonspecifically in tumors through the enhanced permeability and retention (EPR) effect, previous studies using nanoparticles to deliver chemotherapeutics or siRNA demonstrated that attachment of cell-specific targeting ligan...

Biokinetics data of lung-administered PEI F25-LMW/siRNA polyplexes within different lung compartments are presented. Thereby, at three different timepoints (1 h, 3 h, 8 h), the data was determined by calculations to the 32P-radioactivity in the whole mouse body. Additionally, data was optimized to the available PEI F25-LMW/siRNA polyplexes in the target organ and therefore normalized to the sum...

BACKGROUND Polyethylenimine (PEI) is one of the most efficient and versatile non-viral vectors available for gene delivery. Despite many advantages over viral vectors, PEI is still limited by lower transfection efficiency compared to its viral counterparts. Considerable investigation is devoted to the modification of PEI to incorporate virus-like properties to improve its efficacy, including th...

Polyethylenimine (PEI) is considered to be a promising non-viral gene delivery vector. To solve the toxicity versus efficacy and tumor-targeting challenges of PEI used as gene delivery vector, we constructed a novel non-viral vector DR5-TAT-modified Pluronic-PEI (Pluronic-PEI-DR5-TAT), which was based on the attachment of low-molecular-weight polyethylenimine (LMW-PEI) to the amphiphilic polyme...

Phenylboronic acid-modified PEI was prepared by the reaction of 1800 Da PEI with 4-(bromomethyl)phenylboronic acid. It is much more effective than unmodified PEI for gene delivery. The covalently incorporated boronic acid groups achieve the greatly enhanced gene delivery efficiency partially through improving condensation ability to DNA, and partially through facilitating cell uptake due to int...

S mall interfering RNA (siRNA)-mediated RNA interference (RNAi) has received considerable attention since the discovery of the RNAi mechanism in 1998. RNAi holds great promise in molecular therapy of intractable and genetic-related human diseases by silencing the target mRNA (mRNA). 5 For systemic siRNA delivery, naked siRNA is vulnerable to serum and endogenous nuclease degradations. Furthermo...

Polyethylenimine (PEI) has been known as an efficient gene carrier with the highest cationic charge potential. High transfection efficiency of PEI, along with its cytotoxicity, strongly depends on its molecular weight. To enhance its gene delivery efficiency and minimize cytotoxicity, we have synthesized small cross-linked PEI with biodegradable linkages and evaluated their transfection efficie...

25 kDa branched polyethylenimine (PEI) has successfully been used for in vitro and in vivo gene delivery approaches, but it is cytotoxic. Smaller PEIs are usually noncytotoxic but less efficient. In order to enhance the gene delivery efficiency and minimize cytotoxicity of PEI, we explored to synthesize cross-linked PEIs with degradable bonds by reacting amines of small branched 2000 Da PEI wit...

Small interfering RNAs (siRNA) have enabled novel, specific, and efficient treatment of diseases, including cancer. To obtain sufficient well-controlled intracellular delivery, a drug delivery system is essential. Herein for siRNA, composed cationic biodegradable nanogels, presented. P(DEAEMA-co-HEMA-g-PEGMA) nanogels with varying ratios 2-diethyl aminoethyl methacrylate (DEAEMA) 2-Hydroxyethyl...

In recent years, gene therapy has made tremendous progress in the development of disease treatment. Among them, siRNA offers specificity silencing, ease synthesis, and short period, been intensively studied worldwide. However, as hydrophilic polyanion is easily degraded vivo poorly taken up into cells so, benefits its powerful silencing ability will not be realized until better carriers are dev...