Degenerative Disc Disease (DDD) is a prevalent musculoskeletal disorder associated with lower back pain that typically occurs age but can also be precipitated by other factors. At present, the available treatments such as physical therapy, analgesics, anti-inflammatory drugs, and surgical interventions are aimed at addressing symptoms of disease not degeneration process. Among various biologica...

Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchymal stem cells-based therapy for disc degeneration. In vitro assays demonstrated that co-culturing...

A method of percutaneous aspiration of the nucleus pulposus of lumbar disks is described as performed in four pigs and three cadavers. Through a lateral oblique percutaneous approach, 80-100% of the nucleus pulposus was removed in each of the pigs; approximately 30% of the cadaver disk was removed. A unique aspiration probe and the introducing set as well as the technique are described. The imp...

BACKGROUND The aim of this study is to identify which possessed the best stem-cell-like characteristics in 3 kinds of cell in human degenerative intervertebral disc: NPSCs (nucleus pulposus-derived stem cells), AFSCs (annulus fibrosus-derived stem cells), or CESCs (cartilage endplate-derived stem cells). METHODS We separated the disc samples obtained from 15 surgically treated patients with d...

Cellular interactions with the extracellular matrix are key factors regulating cell survival, differentiation, and response to environmental stimuli in cartilagenous tissues. Much is known about the extracellular matrix proteins in the intervertebral disc (IVD) and their variations with region, age, or degenerative state of the tissue. In contrast, little is known of the integrin cell surface r...

Background: A large proportion of patients presenting with lumbar disc disease are opium addicts in our region. It was, therefore, hypothesized that morphine might affect the intervertebral disc. We investigated the histological findings of morphine dependency on intervertebral discs in rat. Methods: Forty NMRI adult male rats (230-250 g) placed on ordinary diet and received aqueous solution of...

In general, the modern literature pays attention to issues of spine pathology and intervertebral discs. A significant percentage vertebral disorders - scoliosis, osteochondrosis, spinal disc herniation, etc., occur as a result exposure various factors manifest in changes The aim our work was study at ultrastructural level features pathomorphological manifestations structural components end seve...