Fluorescence in situ hybridization (FISH) technique was used to detect rDNA sites in chickpea and mungbean. In chickpea, the rDNA sites were detected on three pairs of chromosomes. Among these three pairs of chromosomes, one pair exhibited both 25S rDNA and 5S rDNA sites, while in case of other two pairs of chromosomes the 25S rDNA and 5S rDNA sites were located separately on separate pair of c...

In this work we present a new karyotype for Rhipidomys Tschudi, 1845 (Cricetidae, Rodentia) from Brazil. Our chromosome analyses included GTG- and CBG-banding patterns, the localization of the nucleolus organizer regions after silver staining (Ag-NORs) and fluorescence in situ hybridization (FISH) with a telomere probe. The new karyotype is composed of 44 chromosomes and has a fundamental numbe...

Histone acetylation and deacetylation are connected with transcriptional activation and silencing in many eukaryotic organisms. Gene families for enzymes that accomplish these modifications show a surprising multiplicity in sequence and expression levels, suggesting a high specificity for different targets. We show that mutations in Arabidopsis (Arabidopsis thaliana) HDA6, a putative class I hi...

Cytogenetic studies were performed on two sympatric species of Characidium, C. gomesi and C. cf. zebra, from the Grande River basin, Minas Gerais State, Brazil. Although both species had a chromosome number of 50 with a karyotype exclusively consisting of metaand submetacentric chromosomes, interspecific diversity was detected concerning the size of the two first chromosome pairs of the karyoty...

We carried out a high-resolution ultrastructural analysis of the nucleolus in mouse P815 cells by combining specific DNA and RNA staining, anti-fibrillarin immunolabeling, contrast enhancement by energy filtering TEM and phosphorus mapping by ESI to visualize nucleic acids. We demonstrated that specifically contrasted DNA, fibrillarin and phosphorus overlap within the nucleolar dense fibrillar ...

In the chicken, the nucleolus organizer regions, or sites of the genes encoding 18S, 5.8S, and 28S ribosomal RNA (rRNA), map to one pair of microchromosomes that can be identified by silver nitrate cytochemistry. This nucleolar organizer chromosome also contains the major histocompatibility complex. Chickens aneuploid for this chromosome have been identified and reproduced for over seven genera...

The present study reports the karyotype, C-banding, and nucleolar organizer regions (NORs) of 6 Sus scrofa (Linnaeus, 1758) males from Anatolia. The karyotype of S. scrofa comprised (2n) 38 chromosomes, the number of chromosomal arms (FN) was 64, and the number of autosomal arms (FNa) was 60. C-positive regions appeared to be restricted to the centromeric regions of autosomes 1, and 13-18, and ...

Changes in the structure of the nucleolus during the cell cycle of the Chinese hamster cell in vitro were studied. Quantitative electron microscopic techniques were used to establish the size and volume changes in nucleolar structures. In mitosis, nucleolar remnants, "persistent nucleoli," consisting predominantly of ribosome-like granular material, and a granular coating on the chromosomes wer...