The use of new flocked swabs, compared to kit swabs, enhanced the ability of three commercial nucleic acid amplification tests to detect low levels of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids when the organisms were diluted in a universal transport medium as mocked specimens.

BACKGROUND Nucleic acid amplification (NAA) testing for Mycobacterium tuberculosis (MTB) offers improved diagnostic accuracy, compared with smear microscopy, in differentiating MTB from other mycobacteria. We aimed to evaluate the reliability and projected impact of NAA testing in patients with acid-fast bacilli (AFB) smear-positive respiratory samples. METHODS We identified a retrospective c...

west nile virus (wnv) is an important zoo-notic agent having a wide host range. due to its emergence with increased virulence in a wide geographical range, its monitoring becomes im-perative. development of more rapid and sensitive molecular techniques for instance reverse trans-criptase-polymerase chain reaction (rt-pcr), reverse transcription loop-mediated isothermal amplification (rt-lamp) a...

of the Dissertation Device Engineering for Infectious Disease Diagnosis using Isothermal DNA Amplification and Lateral Flow Detection By Kristina Roskos Keck Graduate Institute of Applied Life Sciences: 2013 Technologies that enable infectious diseases diagnosis in low-resource settings could greatly facilitate effective treatment and containment of such diseases. Nucleic acid amplification tes...

Currently, no consensus exists on assay formats, labels, or detection reactions for nucleic acid assays. New labels continue to be developed and tested, and recent candidates include acetate kinase, firefly luciferase, and genes for enzymes. An additional trend is toward nonamplification strategies (e.g., branched chain and dendrimer type assays) as alternatives to the popular PCR and related a...

The World Health Organization (WHO) international standard (IS) for hepatitis B virus (HBV) DNA for use in nucleic acid amplification assays was characterized by determining the complete genome sequence, which was assigned genotype A. This information will aid the design, development, and evaluation of HBV DNA amplification assays.

OBJECTIVE To compare gonorrhoea detection by self taken vulvovaginal swabs (tested with nucleic acid amplification tests) with the culture of urethral and endocervical samples taken by clinicians. DESIGN Prospective study of diagnostic accuracy. SETTING 1 sexual health clinic in an urban setting (Leeds Centre for Sexual Health, United Kingdom), between March 2009 and January 2010. PARTICI...

Bacteria of the genus Chlamydia belong to order Chlamydiales, within family Chlamydiaceae. These intracellular parasites have a different biphasic reproductive cycle than other bacteria. The important Chlamydiaceae are trachomatis, Chlamydophila pneumoniae and psittaci. trachomatis Clamydophila primary human pathogens. is transmitted by sexual contact. It causative agent LGV (lymphogranuloma ve...

A unique fluorogenic nucleic acid probe has been devised, called the fluorogenic bidirectional displacement probe, which can serve as both the primer and the signal indicator of amplification products for the development of the real-time isothermal DNA amplification and its visual detection of products with high sensitivity and specificity.

Detection of West Nile virus (WNV) by nucleic acid amplification technology (NAAT) is used widely to screen blood and organ donations in areas where WNV is endemic. We report a false-positive result of a WNV transcription-mediated amplification assay (TMA) in a patient with viremia that was caused by Usutu virus, a mosquito-borne flavivirus.