A study was conducted to determine some of the factors affecting the P requirement for the biodegradation of p-nitrophenol, phenol, and glucose by Pseudomonas and Corynebacterium strains. Mineralization of glucose was rapid and the Pseudomonas sp. grew extensively in solutions with 5 and 10 mM phosphate, but the rate and extent of degradation were low and the bacterial population never became a...

A bisubstrate kinetic analysis of UDP-glucuronyltransferase (EC 2.4.1.17) has been carried out in forward and reverse directions with p-nitrophenol as aglycone. Reciprocal plots of initial rates of activity indicated that the kinetics followed a sequential mechanism. Product inhibition studies, using UDP and p-nitrophenylglucuronide as inhibitors of the forward reaction, gave a pattern of two c...

1. A new and rapid continuous assay of rat liver microsomal UDP-glucuronyltransferase (EC 2.4.1.17) has been developed. It is based on measurement of UDP production from UDP-glucuronate during the glucuronidation reaction; UDP production was continuously measured by coupling it to the conversion of NADH into NAD+ through pyruvate kinase and lactate dehydrogenase. This assay is independent of th...

A whole cell-based amperometric biosensor for highly selective, sensitive, rapid, and cost-effective determination of the organophosphate pesticides fenitrothion and ethyl p-nitrophenol thio-benzene phosphonate (EPN) is discussed. The biosensor comprised genetically engineered p-nitrophenol (PNP)-degrading bacteria Pseudomonas putida JS444 anchoring and displaying organophosphorous hydrolase (O...

Adsorption of 1-Butanol, Phenol, and P-nitrophenol on activated carbon havebeen studied. The results were used to testifying the validity of different modelsbased on thermodynamic principles: Freundlich, Langmuir and Henry isotherms.The maximum capacity of adsorbent for three compounds was determined, Theconstants of equilibrium were determined by Freundlich isotherm and virialisotherm expressi...

The design and synthesis of novel cyanopropyl polysilsesquioxane hollow spheres lead to production of a highly active and stable catalyst in the reduction of 4-nitrophenol catalyzed by Au nanoparticles.