What appears to be a new class of phospholipids has been isolated from dog brain in the form of complexes with a substituted phthalanilide. The complexes were extracted by chloroform-methanol and purified by countercurrent distribution in solvent systems containing water, chloroform, methanol, and Freon 113. The binding of the phthalanilide congener to lipids has some ionic character. Cations s...

Creatine kinase (EC 2.7.3.2) activity can be measured fluorometrically by coupling the reaction product, creatine, to ninhydrin at alkaline pH. We have automated this procedure to develop an accurate and precise method, which requires 0.1 ml of sample and can be performed at 60 tests per hour. By using an abbreviated incubation time and a dia!yzer in the iiay, we have extended the analytical ra...

In this manual fluorometric method, blood samples are used that have been impregnated on the filter paper, a convenient collection technique that is widely used to screen newborns for phenylketonuria. The modified procedure, based on the method of McCaman and Robins [J. Lab Clin. Med. 59, 885 (1962)], includes elution of phenylalanine from specimens on filter paper, removal of proteins by preci...

Spiro-N,N-ketal 5, consisting of a phthaloperine heterocyclic ring and a naphtha[1,8-ef][1,4]diazepine ring, was obtained along with spiro-N,N-ketal 2 via 2,2-condensation in the reaction of ninhydrin with naphthalene-1,8-diamine. Their molecular structures were elucidated by X-ray crystal structural analysis. Aside from these spiro compounds, the diazapleiadiene compound 3 formed by 1,2-conde...

The reaction between bis-ninhydrin resorcinol and benzyl-tri-methyl-ammonium fluoride in ethanol has produced the title compound, 2C10H16N(+)·2C24H13O8 (-)·1.5H2O, which contains a unique centrosymmetric supra-molecular dimeric entity, where two deprotonated ligands are held together via two strong and short [O⋯O = 2.4395 (13) Å] [O-H-O](-) bonds of the type negative charge-assisted hydrogen bo...

SUZUKI, M., OSAWA, S. and HIRANO, M. A Lyeium Chinense Miller Component Inducing Ovulation in Adult Female Rabbits. Tohoku J. exp. Med., 1972, 106 (3), 219-231Intravenous injection of the crude extract from Lycii folium among components of Lycium chinense Miller induced ovulation in the adult female rabbit. Chemical properties of the active component of this crude extract were investigated by d...

Most spectroscopic studies of the reaction products formed by ninhydrin, 1,2-indanedione-zinc (Ind-Zn) and 1,8-diazafluoren-9-one (DFO) when reacted with amino acids or latent fingermarks on paper substrates are focused on visible absorption or luminescence spectroscopy. In addition, structural elucidation studies are typically limited to solution-based mass spectrometry or liquid nuclear magne...

Dowex 50. The resin, kindly supplied by Dow Chemical International Ltd., Midland, Michigan, U.S.A., was stated to be of nominal 12% cross-linking and 200-400 mesh. Sodium phosphate buffer pH 6-8, 0.1M. This buffer was adjusted to pH 6-75 in order to delay slightly the emergence of the histidine peak. At the lower pH, histidine emerges separated from and almost mid-way between hydroxylysine and ...

The novel hydrazone-containing thieno[2,3-d]pyrimidine, namely, N′-(1,3-dioxo-1,3-dihydro-2H-inden-2-ylidene)-2-(4-oxo-5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidin-3(4H)-yl)acetohydrazide 4 was synthesized in a very good yield from the reaction of triketoester 1 or ninhydrin 2 with exocyclic acetohydrazide 3 methanol. Good-quality crystals were obtained by recrystallization compound DMF/M...