The cDNA of tomato chloroplastic Cu/Zn-superoxide dismutase was used to construct transgenic tobacco (Nicotiana plumbaginifolia). It was found that the gene expression of Cu/Zn-superoxide dismutase can constitutively be reduced in the transgenic tobacco plants because of double-stranded (dsRNA) expressed in the form of intronspliced hairpin structures and antisense suppression. Furthermore, the...

Transport of the viral genome into the nucleus is an obligatory step in the replication cycle of plant pararetro- and geminiviruses. In both these virus types, the multifunctional coat protein (CP) is thought to be involved in this process. Here, a green fluorescent protein tagging approach was used to demonstrate nuclear import of the CPs of Rice tungro bacilliform virus (RTBV) and Mungbean ye...

In several vegetable species a relationship between the metabolism of active oxygen species and breakage of dormancy has been established. However, scarce information is available on the role of oxidative stress in potato tubers. The purpose of this investigation is to study the effect of CAT genetic modification on the dormancy break of potato tubers. A transgenic approach with partial repress...

We have selected 143 independent Nicotiana plumbaginifolia cell lines that survive in the presence of 5-fluoroorotic acid. These lines show several diverse phenotypes. The majority of these cell lines showed reduced levels of UMP synthase. However, one particular phenotype, which represents 14% of the total independent lines (20 cell lines), showed an unexpected, high level of UMP synthase and ...

The recently published articles in the Iranian Journal of Biotechnology inspired us to write this short communication on nitrogen dioxide NO2-phili...

Transgenic tobacco (Nicotiana plumbaginifolia) seedlings containing the Ca(2+)-sensitive luminescent protein aequorin have been shown to exhibit circadian variations in cytosolic calcium. Concomitant measurements of cytosolic and nuclear calcium show that circadian variations in the cytoplasm are not expressed in the nucleus. To investigate whether all cells of transgenic seedlings contribute e...