The susceptibility of peach GF 305 seedlings and herbaceous plants to five plum pox virus (PPV) isolates from orchards of western Slovakia was investigated. PPV was isolated from diseased plum, apricot and peach trees, and transmitted by chip-budding to peach GF 305. The herbaceous plants were infected by mechanical inoculation. The transmission was analysed by symptomatology and double sandwic...

Pepino mosaic virus (PepMV) poses a worldwide threat to the tomato industry. Considerable differences at the genetic level allow for the distinction of four main genotypic clusters; however, the basis of the phenotypic outcome is difficult to elucidate. This work reports the generation of wild-type PepMV infectious clones of both EU (mild) and CH2 (aggressive) genotypes, from which chimeric inf...

Transgenic expression of proteins in plants is central to research and biotechnology, and, often, it desirable obtain this without altering the nuclear or plastid genomes. Thus, vectors based on plant viruses that infect multiple cells are useful; furthermore, they also advantageous for studies life cycle virus itself. Here, we report development an vector a Turnip vein-clearing (TVCV), tobamov...

peanut plants showing mottling, yellow and necrotic spots on leaves were collected from peanut fields in golestan province. electron microscopic studies revealed the presence of flexuous filamentous particles ca. 700 nm in length, which was suggestive of a potyvirus infection. healthy nicotiana benthamiana plants mechanically inoculated with sap from infected peanut plants showed mottling, down...

Pepper mild mottle virus (PMMoV) is a major viral pathogen of pepper (Capsicum spp.). PMMoV readily mechanically transmitted and seed transmissible. Trisodium phosphate (TSP) treatment commonly used to reduce the level viable in contaminated seed. Ozone efficacious disinfecting fungal-contaminated disrupting bacterial pathogens on various substrates. The purpose this study was evaluate efficacy...

Despite the importance of NHX1 (Na(+)/H(+) exchanger 1) in plant salt tolerance, little is known about its other functions. In this study, intriguingly, it was found that NHX1 participated in plant disease defence against Phytophthora parasitica var. nicotianae (Ppn) in Nicotiana benthamiana. NbNHX1 was originally isolated from N. benthamiana, and characterized. The subcellular localization of ...

Two experimentally important species, Solanum lycopersicon Mill. and Nicotiana benthamiana L., were propagated in vitro under low light (50 micromol m(-2) s(-1)) and transferred to HL (200 micromol m(-2) s(-1)) under a protocol previously developed for grapevine and chestnut. Compared with photooxidative stress parameters already tested in those species, imaging of hydrogen peroxide and superox...

Agrobacterium-mediated transient protein production in plants is a promising approach to produce vaccine antigens and therapeutic proteins within a short period of time. However, this technology is only just beginning to be applied to large-scale production as many technological obstacles to scale up are now being overcome. Here, we demonstrate a simple and reproducible method for industrial-sc...

The requirement for the 17-kDa protein (P17) of Potato leafroll virus (PLRV) in virus movement was investigated in four plant species: potato (Solanum tuberosum), Physalis floridana, Nicotiana benthamiana, and N. clevelandii. Two PLRV P17 mutants were characterized, one that does not translate the P17 and another that expresses a P17 missing the first four amino acids. The P17 mutants were able...

Although cyclic glucans have been shown to be important for a number of symbiotic and pathogenic bacterium-plant interactions, their precise roles are unclear. Here, we examined the role of cyclic beta-(1,2)-glucan in the virulence of the black rot pathogen Xanthomonas campestris pv campestris (Xcc). Disruption of the Xcc nodule development B (ndvB) gene, which encodes a glycosyltransferase req...