One important function of humoral immunity is toxin neutralization. The current view posits that neutralization results from antibody-mediated interference with the binding of toxins to their targets, a phenomenon viewed as dependent only on antibody specificity. To investigate the role of antibody constant region function in toxin neutralization, we generated IgG2a and IgG2b variants of the Ba...

The rapid fluorescent focus inhibition test (RFFIT) is routinely used in the United States to measure rabies virus neutralizing antibodies (rVNA). RFFIT has a long history of reproducible and reliable results. The test has been modified over the years to use smaller volumes of reagents and samples, but requires a 50 μL minimum volume of test serum. To conduct pathogenesis studies, small laborat...

Past research on information technology (IT) security training and awareness has focused on informing employees about security policies and formal sanctions for violating those policies. However, research suggests that deterrent sanctions may not be the most powerful influencer of employee violations. Often, employees use rationalizations, termed neutralization techniques, to overcome the effec...

Assessing the neutralization capability of nonlethal but medically relevant toxins in venom has been a challenging task. Nowadays, neutralization efficacy is evaluated based simply on the survival rates of animals injected with antivenom together with a predefined dose of venom, which can determine potency against neurotoxicity but not validate the capability to neutralize cytotoxin-induced com...

Three defense functions of immunoglobulin A (IgA), immune exclusion, intracellular neutralization, and virus excretion, were assessed in a measles virus model using polarized epithelial cells expressing the polymeric immunoglobulin receptor and monoclonal antibodies against the viral H and F envelope proteins and the internal N protein. Anti-H IgA was the most effective antibody at preventing i...

Kinetic curves for neutralization of adenovirus type 2 with anti-hexon serum revealed no lag periods even when the serum was highly diluted or when the temperature was lowered to 4 degrees C, thus indicating a single-hit mechanism. Multiplicity curves determined with anti-hexon serum displayed a linear correlation between the degree of neutralization and dilution of antiserum. Neutralization va...

Abstract Carbon neutralization is a new way of environmental protection, in which carbon capture the core neutralization. Firstly, preparation method polyanthraquinone/multi-walled nanotube composites (PAQ/MWCNTs) was studied. The were prepared by magnetic stirring and centrifugal separation. working electrode using composite material for capture. A three-electrode system established to dioxide...

OBJECTIVE To verify the presence of anti-rotavirus serotype G9P[5] SIgA and the virus neutralization capacity of milk samples from Brazilian women. METHODS SIgA antibody levels reactive to rotavirus G9 were determined in 30 maternal milk samples by enzyme-linked immunosorbent assay (ELISA) using purified virus suspensions. The samples' capacity to neutralize rotavirus G9P[5] was analyzed usin...

Both legal and illegal forms of digital music consumption continue to evolve with wider adoption of subscription streaming services. With this paper, we aim to extend theory on digital music piracy by showing that the rising controversy and diminishing acceptance of illegal forms of consumption call for new theoretical components and interactions. We introduce a model that integrates insights f...