the objective of the study was to evaluate the cytotoxic effects of aqueous extract of cucumis melo fruit on human prostate cancer cell line (pc-3) using mtt and neutral red assays. the crude aqueous extract of c. melo was prepared by cold maceration method, filtered, concentrated and tested on pc-3 cell line. dose-dependent cytotoxic activities were exhibited on human prostate carcinoma pc-3 c...

Introduction: Skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. The aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. Methods: In this pa...

A neutral red assay involving Vero cells was used to quantitate the cytotoxic activity of verotoxins (VT) produced by Escherichia coli and to investigate changes in titer caused by altering the composition of the cell culture medium. Three variations on medium 199 were investigated: one involved supplementing the medium with 5% fetal bovine serum (FBS), a second was the use of serum-free (SF) m...

Recent dynamical analyses of the Kuiper belt have introduced a rigorous classification scheme, determined the mean orbital plane, and identified “Core” and “Halo” populations as a function of inclination with respect to this plane (Elliot, J.L., Kern, S.D., Clancy, K.B., Gulbis, A.A.S., Millis, R.L., Buie, M.W., Wasserman, L.H., Chiang, E.I., Jordan, A.B., Trilling, D.E., Meech, K.J., 2005. Ast...

Ever since vagotomy became an important part of modern peptic ulcer surgery, investigators have searched for a test that would reliably indicate the completeness of vagus nerve section. The insulin hypoglycemia test, originally advocated by Hollander [8], has remained the most widely utilized test for the completeness of vagotomy, but despite several modifications it’s reliability has been ques...

Este artículo presenta el prototipo del diseño de la Red Abierta, Libre y Neutral para la ciudad de Cúcuta, la cual es una alternativa para el intercambio de información sin costo ante las opciones que ofrecen las empresas de telecomunicaciones en la ciudad. Se define el acuerdo entre iguales que permite la sana convivencia de los miembros, así como contenidos y servicios. El diseño físico y ló...

cadmium oxide rhombus-shaped nanostructure was synthesized using hydrothermal process followed by heating treatment. clearly, x-ray diffraction pattern demonstrated the formation of cdo crystalline phase. scanning electron microscopy (sem) showed that the obtained rhombus-like structure is composed of nanoparticles with the average size of 29 nm. in addition, we evaluated adsorption of organic ...

AIM The present study was conducted to survey the potential cytotoxic influence of freeze-dried aqueous extract of its fruits on gastrointestinal cell lines, namely AGS (human gastric carcinoma) and KYSE30 (human esophageal squamous cell carcinoma. BACKGROUND Rosemary (Rosmarinus officinalis) is a wild medicinal plant shown to have anticancer activity. Carnosic and rosmarinic acids are compou...

BACKGROUND The study was aimed at investigating the effect of various solvents on the absorption spectra of Neutral Red, a dye belonging to the quinone-imine class of dyes. The solvents chosen for the study were water, ethanol, acetonitrile, acetone, propan-1-ol, chloroform, nitrobenzene, ethyleneglycol, acetic acid, DMSO and DMF. RESULTS The results have shown that the absorption maxima of d...