Four laccase isozymes (LCC1, LCC2, LCC3 and LCC4) synthesized by Pleurotus ostreatus strain V-184 were purified and characterized. LCC1 and LCC2 have molecular masses of about 60 and 65 kDa and exhibited the same pI value (3.0). Their N termini were sequenced, revealing the same amino acid sequence and homology with laccases from other microorganisms. Laccases LCC3 and LCC4 were characterized b...

Content Page

The electrophoresis of hydrophobic proteins underwent signi®cant development during the last decade. In native gel electrophoresis, the induced charge shift, the involvement of mild detergents as well as a constant ionic strength in the electrophoresis, overcame the dif®culty of the low solubility and aggregation of the hydrophobic proteins, and critically improved the results of their native P...

Self-association of histones H1 from calf thymus and from sperm of the marine worm Chaetopterus variopedatus was studied on native and glutaraldehyde cross-linked molecules by PAGE and by salt-induced turbidity measurements. Multiple polymers were generated by native sperm histone H1-like after glutaraldehyde cross-linking while the same treatment on its lysine- or arginine-modified derivatives...