نتایج جستجو برای: nasba method

تعداد نتایج: 1630253  

Journal: :Blood 2003
Giuseppe Gerna Daniele Lilleri Fausto Baldanti Maria Torsellini Giovanna Giorgiani Marco Zecca Piero De Stefano Jaap Middeldorp Franco Locatelli M Grazia Revello

In the search for better protocols of preemptive therapy of human cytomegalovirus (HCMV) infection in hematopoietic stem cell transplant (HSCT) recipients, we conducted a randomized trial comparing antigenemia with the nucleic acid sequence-based assay (NASBA) for determination of HCMV immediate-early messenger RNA (IEmRNA) as the guiding assay for initiation of pre-emptive antiviral treatment....

Journal: :Molecular pathology : MP 1999
D P Hayes A A Brink M B Vervoort J M Middeldorp C J Meijer A J van den Brule

AIMS To examine the expression of Epstein-Barr virus (EBV) transcripts encoding proteins homologous to important human proteins in diverse EBV associated diseases. The proteins were: BHRF1 (homologous to Bcl-2), BDLF2 (homologous to cyclin B1), BARF1 (homologous to intercellular cell adhesion molecule 1 (ICAM-1)), and BCRF1 (viral IL-10 (vIL-10), homologous to human IL-10 (hIL-10)). METHODS S...

Journal: :Journal of clinical microbiology 2003
Christine C Ginocchio Marti Kemper Kathleen A Stellrecht Donald J Witt

The analytical performance of the NucliSens HIV-1 QT assay, a highly sensitive test based on nucleic acid sequence-based amplification technology, was evaluated in a multicenter trial. Assay specificity was evaluated with 502 plasma (EDTA) specimens from human immunodeficiency virus type 1 (HIV-1)-seronegative volunteer donors. No HIV-1 RNA was reported in any of the donor specimens. Analytical...

Journal: :Blood 2000
R F Franco E de Jonge P E Dekkers J J Timmerman C A Spek S J van Deventer P van Deursen L van Kerkhoff B van Gemen H ten Cate T van der Poll P H Reitsma

Triggering of the tissue factor (TF)-dependent coagulation pathway is considered to underlie the generation of a procoagulant state during endotoxemia. To determine the in vivo pattern of monocytic TF messenger RNA (mRNA) expression during endotoxemia, 10 healthy volunteers were injected with lipopolysaccharide (LPS, 4 ng/kg) and blood was collected before and 0.5, 1, 2, 3, 4, 6, 8, and 24 hour...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه لرستان - دانشکده علوم پایه 1389

abstract part one: the electrode oxidation potentials of a series of eighteen n-hydroxy compounds in aqueous solution were calculated based on a proper thermodynamic cycle. the dft method at the level of b3lyp-6-31g(d,p) was used to calculate the gas-phase free energy differences ,and the polarizable continuum model (pcm) was applied to describe the solvent and its interaction with n-hydroxy ...

2008
Dany Morisset David Dobnik Sandrine Hamels Jana Žel Kristina Gruden

We have developed a novel multiplex quantitative DNA-based target amplification method suitable for sensitive, specific and quantitative detection on microarray. This new method named NASBA Implemented Microarray Analysis (NAIMA) was applied to GMO detection in food and feed, but its application can be extended to all fields of biology requiring simultaneous detection of low copy number DNA tar...

Journal: :International Journal of Clinical and Experimental Medical Sciences 2023

Malaria is a serious public health problem worldwide. Globally concerted efforts are underway to control and eliminate it. Despite recent slowdown, substantial achievements have been recorded in the last 20 years. However, its eradication requires successful elimination of all Plasmodium parasites among symptomatic, asymptomatic, sub-microscopic infections. This review aimed at assessing...

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