Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resol...

DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic acid molecule. Nanopore detection and characterization of single molecules represents a new meth...

Next-generation sequencing methods based on nanopore technology have recently gained considerable attention, mainly because they promise affordable and fast genome sequencing by providing long read lengths (5 kbp) and do not require additional DNA amplification or enzymatic incorporation of modified nucleotides. This permits health care providers and research facilities to decode a genome withi...

A single molecule of Deoxyribonucleic acid (DNA) is being sequenced by using an innovative concept of Nanopore. The orientation of nanopore define the channel of ions through the pore comes into contact with nucleotide, the permutation and the transition kinematics of each molecule of DNA. The divergence of electrical signal observed when the four chemical bases adenine (A), guanine (G), cytosi...

Nanopore-based single-molecule sequencing techniques exploit ionic current steps produced as biomolecules pass through a pore to reconstruct properties of the sequence. A key task in analyzing complex nanopore data is discovering the boundaries between these steps, which has traditionally been done in research labs by hand. We present an automated method of analyzing nanopore data, by detecting...

The synthesis of bulk amounts of high quality single-walled carbon nanotubes (SWNTs) is accomplished by optimizing the chemical compositions and textural properties of the catalyst material used in the chemical vapor deposition (CVD) of methane. A series of catalysts are derived by systematically varying the catalytic metal compounds and support materials. The optimized catalysts consist of Fe/...

We show that a semiconductor membrane made of two thin layers of opposite (n- and p-) doping can perform electrically tunable ion current rectification and filtering in a nanopore. Our model is based on the solution of the 3D Poisson equation for the electrostatic potential in a double-cone nanopore combined with a transport model. It predicts that, for appropriate biasing of the membrane-elect...

Using nanopores to sequence DNA rapidly and at a low cost has the potential to radically transform the field of genomic research. However, despite all the exciting developments in the field, sequencing DNA using a nanopore has yet to be demonstrated. Among the many problems that hinder development of the nanopore sequencing methods is the inability of current experimental techniques to visualiz...

Electrode/nanopore structures hold promise towards the fabrication, functionalization and control of nanopore sensor performance. This includes "simple" one-electrode structures, where an additional membrane electrode is used to control the electrostatic properties of the nanopore, but also more sophisticated two-electrode junction devices, integrating tunneling detection with nanopore-based si...

One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using the 52 SNP-plex assay developed by the SNPforID consortium. All but one of the loci were correctly genotyped. Several SNP loci were identified...