Objectives: To understand that adipose stem cell exosome (ASCE) can be a paradigm shift in regenerative aesthetics & therapeutics. know ASCE make scalp rejuvenation and hair loss prevention. Introduction: Exosomes are nano-sized (30 – 200 nm diameter) lipid bilayered vesicles secreted by most types. Adipose Stem Cell-derived emerging as promising cell-free therapy for the treatment of rejuvenat...

We report the straightforward synthesis of dual stimuli-responsive nano-objects using a RAFT-mediated polymerization-induced self-assembly process in water. The key to complex and tunable responsiveness was copolymerization N-cyanomethylacrylamide, providing upper critical solution temperature-type polymers, with pH-sensitive monomer (acrylic acid, AA). Not only core block length but also AA co...

Despite the promise of RNA interference (RNAi) and its potential, e.g. for use in cancer therapy, several technical obstacles must first be overcome. The major hurdle of RNAi-based therapeutics is to deliver nucleic acids across the cell's plasma membrane. This study demonstrates that exosome vesicles derived from humans can deliver short interfering RNA (siRNA) to human mononuclear blood cells...

Cell to cell communication is essential for the coordination and proper organization of different cell types in multicellular systems. Cells exchange information through a multitude of mechanisms such as secreted growth factors and chemokines, small molecules (peptides, ions, bioactive lipids and nucleotides), cell-cell contact and the secretion of extracellular matrix components. Over the last...

The present study was designed to develop and compare acyclovir containing nano-vesicular liposomes and niosomes based on cholesterol, soya L-alpha-lecithin and nonionic surfactant, span 20. The effort was made to study in vitro whether acyclovir-loaded nanovesicles could sustain the release of the drug by increasing residence time and thus, acyclovir could reduce its dose-related systemic toxi...

A unilamellar polymeric vesicle is a self-assembled structure of a block copolymer that forms a spherical single bilayer structure with a hydrophobic interlayer and a hydrophilic surface. Due to their enhanced colloidal stability and mechanical property, controllable surface functionality, or tunable membrane thickness, polymeric vesicles are useful in nano and bio-science, providing potential ...

Single particle characterization has become increasingly relevant for research into extracellular vesicles, progressing from bulk analysis techniques and first-generation to comprehensive multi-parameter measurements such as nano-flow cytometry (nFCM). nFCM is a form of flow that utilizes instrumentation specifically designed nano-particle analysis, allowing thousands EVs be characterized per m...

background: extracellular vesicles are particles ranged from 30 nm to 5µm and subcategorized into three groups; exosomes, microvesicles and apoptotic bodies, each of which have different biological impact. lack of a standard method for the detection and isolation of mvs has led to a challenging issue that is a worth considering. in this study, we isolated mvs from the conditioned medium of uc-m...