A 6.09-kb plasmids vector pOri253 was constructed from the plasmid pIL253 (5.2 kb) and a 0.89-kb fragment of oriColE1 from pBluescript II KS. The bifunctional plasmid pOri253 conferred erythromycin resistance in both Escherichia coli and Enterococcus faecalis. It has a unique site for each of EcoRI, BamHI, SalI and PstI derived from pIL253. The plasmid is quite stable in E. faecalis JCM8726 whe...

Bacteriophages (prokaryotic viruses) are favourite model systems to study DNA replication in prokaryotes, and provide examples for every theoretically possible replication mechanism. In addition, the elucidation of the intricate interplay of phage-encoded replication factors with 'host' factors has always advanced the understanding of DNA replication in general. Here we review bacteriophage rep...

In man the nitrogen in position 7 is derived from glycine (3). Similar mechanisms are involved in the formation of the nucleic acid guanine in yeast (4) and hypoxanthine in pigeon liver homogenates (5). The earlier demonstration that L-serine is extensively converted to glycine (6) and, presumably, a 1 carbon intermediate provides ground for belief that the P-carbon atom of serine may be a prec...

This paper defends the Euro from point of view Austrian Economics. Folloming Mises and Hayek it demonstrates is acting as a proxy gold standard disciplining polititians putting limit to growth welfare state. Key words: Gold standard, fixed flexible exchange rates, state crisis, competition among monetary areas, keynesianism, monetarism, School. JEL Classification: N15, N45, O53, P11, X10. Resum...

Uric acid has previously been shown to act as a water-soluble antioxidant. Although the antioxidant activity of uric acid has been attributed to its ability to scavenge free radicals, the one-electron uric acid oxidation product of such a scavenging reaction has not been detected. It order to determine whether a free radical metabolite of uric acid could be formed via one-electron redox process...