Tuberculosis control is a priority for the Ministry of Health policies in Brazil. In the present work, the detection of Mycobacterium tuberculosis by the Polymerase Chain Reaction (PCR) was standardized, and the laboratory diagnosis of pulmonary tuberculosis was evaluated comparing baciloscopy, culture and PCR tests. The study was carried out with 117 sputum samples from different patients susp...

drug resistance (especially multiple drug resistance) in mycobacterium tuberculosis makes global concerns in treatment and control of tuberculosis. rapid diagnosis of drug resistant strains of the bacteria has vital importance in the prognosis of the disease. the aim of this study was to identify the mutations responsible for drug resistance in mycobacterium tuberculosis strains derived from pa...

In summary, in order to control tuberculosis, we require a rapid diagnosis of TB patients, who act as the source of disease; and if remain untreated, these patients can spread the disease throughout the population. Therefore, we need to introduce the new approach for quick and accurate identification of Mycobacterium tuberculosis, and CYP141 gene is a new approach for rapid and accura...

OBJECTIVES Isoniazid (INH) is one of the main first line drugs used in treatment of tuberculosis and development of resistance against this compound can result in serious problems in treatment procedures. Resistance to INH is mediated mainly by mutation in KatG gene that is coded for the catalase enzyme. The proportional method for detection of INH-resistance is time consuming due to the slow g...

Molecular markers provide an important method for detecting tuberculosis (TB) transmission that has been followed by rapid progression to active disease. Restriction fragment length polymorphism (RFLP) typing of Mycobacterium tuberculosis has been used to confirm outbreaks, through the demonstration of shared isolates. In population-based studies, it has therefore been inferred that patients wi...

Sixty-eight ancestral Mycobacterium tuberculosis isolates were previously identified by using the tuberculosis-specific deletion 1 (TbD1) PCR and mycobacterial interspersed-repetitive-unit-variable-number tandem repeat (MIRU-VNTR) typing (Y. J. Sun, R. Bellamy, A. S. G. Lee, S. T. Ng, S. Ravindran, S.-Y. Wong, C. Locht, P. Supply, and N. I. Paton, J. Clin. Microbiol. 42:1986-1993, 2004). These ...

OBJECTIVE To compare three methods: Biochemical tests, high-performance liquid chromatography (HPLC) and polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP), for the identification of mycobacteria, and to perform a cost-benefit analysis to define an optimum identification algorithm. MATERIAL AND METHODS One-hundred-and-seven mycobacteria isolates were identified by ...

An evaluation of the utility of rep PCR typing compared to the 15 loci discriminatory set of MIRU-VNTR was undertaken. Twenty-nine isolates of Mycobacterium tuberculosis from patients were examined. Genomic DNA was extracted from the isolates by standard method. The number of copies of tandem repeats of the 15 MIRU-VNTR loci was determined by PCR amplification and agarose gel electrophoresis of...

A study set of 180 Mycobacterium tuberculosis and Mycobacterium bovis isolates having low copy numbers of IS6110 were genotyped using the recently introduced method based on the variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR). The results were compared with results of the more commonly used methods, IS6110 restriction fragment length polymorphism (RFLP)...

BACKGROUND A large and unsuspected tuberculosis outbreak involving 18.7% of the total of the tuberculosis cases studied, was detected in a population-based molecular epidemiological study performed in Zaragoza (Spain) from 2001 to 2004. METHODS The Mycobacterium tuberculosis drug-susceptible strain, named MTZ strain, was genetically characterized by IS6110-RFLP, Spoligotyping and by MIRU-VNTR...