Poly (ADP-ribose) polymerases (PARP) 1-3 are well-known multi-domain enzymes, catalysing the covalent modification of proteins, DNA, and themselves. They attach mono- or poly-ADP-ribose to targets using NAD+ as a substrate. Poly-ADP-ribosylation (PARylation) is central important functions PARP enzymes in DNA damage response nucleosome remodelling. Activation happens through binding via zinc fin...

The value of dietary exogenous enzymes in promoting growth and efficiency of feed utilization is well recognized in poultry industry. In a case of high-cost yellow corn, several feed producers are choosing to replace yellow corn with other ingredients that have lower nutritional value like triticale, wheat, barley or sorghum. These crops are rich in non-starch polysaccharides (NSPs), and there ...

Background and Aims: In this study we improved the purification of immunoglobulins from equine antiserum raised against the venom of 6 types of scorption species. Caprylic acid (octanoic acid), a fatty acid, was found to have no effect on the activity of the enzymes pepsin, which is used in antivenom purification to digest Fc fragment of immunoglobulins to obtain F(ab´)2. Materials and Methods...

UNLABELLED AiO (All in One) is a program for Windows, that combines typical DNA/protein features such as plasmid map drawing, finding of ORFs, translate, backtranslate and high quality printing with a number of databases. These databases allow the management of oligonucleotides, oligonucleotide-manufacturers, restriction enzymes, structural DNA and program users in a multi-user/multi-group envi...

Nickel(II) bis(diphosphine) complexes that contain positioned bases in the second coordination sphere have been found to catalyze the reduction of O(2) with H(2) to selectively form water. The complexes also serve as electrocatalysts for the reduction of O(2) with the addition of a weak acid. In contrast, a closely related nickel diphosphine complex without the positioned bases is catalytically...

Inflammation of pancreatic parenchyma is said to be pancreatitis. It can be acute, presenting as emergency with short history or chronic as a continuation of acute with a long history. Autodigestion is responsible for pancreatitis on background of premature activation of pancreatic enzymes within the pancreas. Acute Pancreatitis is acute inflammation of the pancrease which can be mild to modera...

We describe a multimodal microscope for visualizing processive enzymes moving on immobilized substrates. The instrument combines interference reflection microscopy (IRM) with multi-wavelength total internal reflectance fluorescence (TIRFM). can localize quantum dots precision of 2.8 nm at 100 frames/s, and was used to image the dynamics cellulase, Cel7a interacting surface-immobilized cellulose...