Background: MTT assay is a colorimetric test to evaluate cell metabolic activity of living cells via reduction the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide insoluble formazan crystals by mitochondrial activities. The tetrazolium dye thought occur NADPH-dependent oxidoreductase enzymes in cytosol. used measure cytotoxicity or cytostatic generally plant and chemical compounds ...

Mixing the residue in the gut has been studied in 24 normal subjects using five different types of radio-opaque pellets. By giving single doses of different pellets to the subjects on three successive days and measuring their appearances in the stool by radiographs evidence of mixing was found in 83% of studies. Mean transit time (MTT) of markers through the gut was measured from the single dos...

The hydrogen acceptor 3-(4,5-dimethylthiazol-2-yl)-2,5 dipheny Itetrazolium bromide (Mil) is commonly utilized to estimate cellular viability in drug screening protocols. The present investigation was prompted, in part, by observations that reduction of MTT to its colored reaction product, MTT formazan, varied between cell lines and with culture age. A correlation was established between the D-...

Reconstruction and augmentation of the alveolar bone defects pose a challenge for dental surgeons due to its complex structure. The primary objective tissue engineering is regenerate or replace damaged tissues organs including with grafts, cells, biological molecules. 45S5-bioglass (45S5-BG), superior osteoconductive osteoinductive abilities, has been at forefront engineering, regeneration, per...

Green=FM[C], Black=“FM[localAIF]”, Red=FM[oR], Blue=Tmax[oR]. a) FM vs VTF-delay (MTT=4s, dispersion=0s), b) FM vs VTF-dispersion (MTT=4s, delay=0s), c) FM vs tissue MTT (delay=0s, dispersion=0s). Figure 2: In vivo results from a single patient: Top row: local AIF source voxels. a) method1, b) method2. Middle row: MTT maps. c) global AIF, d) method1, e) method2. Bottom row: Tmax maps. f) global...

Principles of echo shifting with a train of observations was used to perform magnetic susceptibility-weighted magnetic resonance imaging with bolus-tracking in 14 patients with spondylotic myelopathy to assess changes in perfusion parameters of the spinal cord before and after decompression surgery for cervical spondylotic myelopathy. The mean transit time (MTT), bolus arrival time (T0), and ti...

This paper describes a critical evaluation of a miniaturised colorimetric assay, using MTT (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) reduction, applied to protozoan viability testing. The toxic substances used were copper, zinc, Triton X-100 (a membrane surfactant) and cycloheximide (an inhibitor of the protein synthesis). The viability assay of the ciliate protozoan Tetr...