fungi produce a wide range of secondary metabolites such as antibiotics, toxins, alkaloids, fatty acids, ketones and alcohols during active cell growth. the present study was aimed to identify secondary metabolites from some penicillium species, using gc-ms. many important compounds such as 3-oxoquinuclidine in penicillium jenseii, formamidine in penicillium pusillum, orcinol and 1,3,8-p-mentha...

multiple sclerosis (ms) is a demyelinating disease of the central nervous system,with unknown etiology in which both genetic and environmental factors are thought to be involved. the hla system provides a set of genetic markers which lend themselves to systematic study. the disease also has variable clinical manifestations, ranging from a relapsing-remitting course to a chronic progressive dise...

background methotrexate is a folic acid antagonist that has been in clinical use for five decades. its use in patients with brain tumors is primarily confined to primary central nervous system lymphoma (pcnsl), in which it is the cornerstone of chemotherapy. objectives a selective and sensitive high-performance liquid chromatography-electrospray ionization-mass spectrometry method was developed...

The subject of this tutorial is protein identification and characterisation by database searching of MS/MS Data. Peptide Mass Fingerprinting is excluded because it is covered in a separate tutorial. Practical aspects of database searching are emphasised, such as choice of sequence database, effect of mass tolerance, and how to identify post-translational modifications. The relationship between ...

The development of a new ion mobility/mass spectrometry instrument that incorporates a multifield drift tube/ion funnel design is described. In this instrument, individual components from a mixture of ions can be resolved and selected on the basis of mobility differences prior to collisional activation inside the drift tube. The fragment ions that are produced can be dispersed again in a second...

System suitability is widely recognized as a critical component of bioanalysis. This paper discusses a generic system suitability test that monitors instrument performance throughout a run when used for liquid chromatography tandem mass spectrometry (LC/MS/MS) in bioanalysis. This system suitability process is designed to ensure that the LC/MS/MS system is performing in a manner that leads to t...