Aberrant DNA methylation has been reported as an important phenotype in acute myeloid leukemia. However the clinical significance of methylation changes has not been clear yet. In this study methylation Specific Melting Curve Analysis (MS-MCA) and real time PCR was used to assess the CDKN2B promoter hyper-methylation and gene expression in 59 Iranian acute myeloid leukemia (AML) patients. The i...

background human telomerase reverse transcriptase (htert) has become an ideal target for development of anticancer therapy. small interfering rnas (sirnas) are very powerful reagents for gene silencing and show promise for cancer gene therapy. however, only a small number of sirnas have been demonstrated to be effective. for gene therapy targeting htert, it is essential to develop a robust syst...

Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite in the. The main objective of this research was to investigate stimulatory effects of sodium nitrate, potassium nitrate, ammonia and urea on the production/generation of the nitrate reductase mRNA in Triticum aestivum plants. The pl...

Background: Nano-particle Titanium Dioxide (TiO2) is a noncombustible, odorless powder that is widely used in different fields of industries. Previous reports showed that chronic exposure to TiO2 adversely impacts the testicular tissue and down-regulates the antioxidant capacity and down-regulates the endocrine status of the testicles. Present study was designed in order to identify the role of...

Background: The herpes simplex virus (HSV) UL41 gene product, virion host shutoff (Vhs) protein, mediates the rapid degradation of both viral and cellular mRNA. This ability suggests that Vhs protein can be used as a suicide gene in cancer gene therapy applications. The recent reports have shown that the degradation of cellular mRNA during herpes simplex infection is selective. RNA containing A...

Abstract Background Messenger RNA sequencing is becoming more common in studies of non-model species and most often used for gene expression-based investigations. However, the method holds potential numerous other applications as well—including analyses alternative splicing, population structure, signatures selection. To maximize utility mRNA data sets, distinct may be combined such by explorin...

Background In vitro maturation (IVM) of human oocytes has acquired increasing attention in infertility treatment with great promise. This technique is an alternative conventional in vitro fertilization-embryo transfer (IVF-ET), and can be reduced the side effects of gonadotropin stimulation such as ovarian hyperstimulation (OHSS). Oocyte maturation is a complex process including cytoplasmic and...

Objective: The goal of this research was to determine the influence of 4 weeks aerobic training on gene expression of tumor necrosis factor alpha (TNF-α), hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in the cardiac tissue of diabetic rats. Materials and Methods: In an experimental study, 30 male wistar rats were partitioned into three groups (n=10), d...