Chromosomal integrity has been known for many years to affect the ability of mouse embryonic stem cells (mESCs) to contribute to the germline of chimeric mice. Abnormal chromosomes are generally detected by standard cytogenetic karyotyping. However, this method is expensive, time consuming, and often omitted prior to blastocyst injection, consequently reducing the frequency of mESC-derived offs...

Cell culture using a 3D method provides Various cell strategies have been developed synthetic or biological materials; most existing publications use many reagents. Bioscaffold from mouse embryonic fibroblast (MEF) enhances attachment, interaction, and production of growth factors. Since bioscaffolds could maintain stimulate pluripotency stem cells, we conducted this study to prove bioscaffold ...

in vitro expansion of mesenchymal stem cell (mscs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. on the other hand, some studies have indicated that bio (6-bromoindirubin-3-oxime) possesses mitogenic effects on cell culture. the objective of the present study was to examine the effect of bio on in vitro expansion and chondrogenic di...

The enzyme P450c17 is required for glucocorticoid, sex steroid, and some neurosteroid biosynthesis. Defective human P450c17 causes sexual infantilism and 46,XY sex reversal but is compatible with life, whereas ablation of the corresponding mouse gene causes embryonic lethality at around E7. Normal mouse embryos express P450c17 protein and activity in the embryonic endoderm at E7. Adult adrenal ...

Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell cult...

Background: The regenerative capacity of the mammalian heart is quite limited. Recent reports have focused on reprogramming mesenchymal stem cells into cardiomyocytes. We investigated whether fibroblasts could transdifferentiate into myocardium.Methods: Mouse embryonic fibroblasts were treated with Trichostatin A (TSA) and 5-Aza-2-Deoxycytidine (5-aza-dC). The treated cells were permeabilized w...