in order to evaluate the effects of phenytoin on the reproductive system of rats in detail, 60 male rats were divided equally into 6 groups: 1-5 received 10 mg/kg/day phenytoin ip for 1,2,3,4 and 5 weeks, respectively. however, group 5 did not receive any phenytoin for the last 5th week. the control group (group 6) did not receive any treatment. the results revealed that phenytoin does not have...

The FertDish features a clinically applicable sperm processing format, and enables high recovery of motile with DNA quality.

The existence of a complex population of mRNA in human sperm is well documented but their role is not yet elucidated. Using discontinuous density gradients, we have isolated high and low motile sperm from the same semen sample. The levels of different transcripts coding for molecules either involved in nuclear condensation (protamines 1 and 2) or in capacitation [endothelial nitric oxide syntha...

sperms are highly specialized cells for delivering dna from male to the ovum. incredibly, wide degree of diversity in sperm morphology in their basic structures i.e. head, middle piece and tail is found across species. differences in terms of overall size of the sperm, shape and number of sperm produced are also incredible. one of the key for this variations or diversity in sperm may be associa...

The purpose of this study was to characterize highly enriched human spermatozoa membrane proteins by two-dimensional electrophoresis and computer image analysis. Sperm membrane proteins were extracted by detergent solubilization from three different preparations: 1) washed semen cells following centrifugation and three wash steps in Ham's F-10 medium (the standard sperm preparation, which is co...

The use of cryopreserved donor spermatozoa for insemination has become necessary to decrease the risk from sexually transmitted infectious diseases. Lower fecundity rates have been reported with this practice. Efforts have been applied to increase success, including identification of those sperm characteristics which correlate with increased fecundity. Data from in-vitro fertilization have reve...

The outcomes of drug treatment for male infertility remain conjectural, with controversial study results. Our pilot study employed a randomized, placebo-controlled, crossover methodology with intention-to-treat analysis. Thirty-three men with idiopathic oligospermia were randomized to start either daily oral lisinopril 2.5 mg (n = 17) or daily oral placebo (n = 16). Lisinopril was found to caus...

background: the etiologic cause in near one third of male factor infertility is unknown. the percentage of men with idiopathic infertility who have been successfully treated by the empirical therapeutic modalities is not high. objective: the aim of this study was to assay the effect of l-carnitine on sperm parameters in patients who needs intracytoplasmic sperm injection (icsi) as a method for ...

Artificial insemination (AI) can help to avoid inbreeding and genetic degeneration for sustaining genetically healthy populations of endangered species in captivity. Collection of a sufficient quantity of viable sperm is an essential first step in the AI process. In the present study, we examined the effects of frequent electroejaculation on semen characteristics in a Siberian tiger. We collect...

When ejaculates from rival males compete for fertilization, there is strong selection for sperm traits that enhance fertilization success. Sperm quantity is one such trait, and numerous studies have demonstrated a positive association between sperm competition and both testes size and the number of sperm available for copulations. Sperm competition is also thought to favor increases in sperm qu...