Background . The study of genetic predictors non-Hodgkin’s lymphomas prognosis is one the most relevant areas oncohematology. It extremely interesting to search for integral markers that reflect important stages tumor pathogenesis. DNA repair system plays key roles in genomic instability. Aberrations microsatellite repeats such as instability (MSI), particular elevated alterations at selected t...

The nonenzymatic reduction of metmyoglobin and methemoglobin by NADH or NADPH in the presence of ethylenediaminetetraacetate can occur at rates greater than most of those previously reported for enzymatic reduction. The reduction rate is considerably enhanced by the mediation of various flavins, including FAD, flavin mononucleotide, and riboflavin. The system is generally more active at pH 6.4 ...

We determined and annotated the complete 2.2-megabase genome sequence of Pyrobaculum aerophilum, a facultatively aerobic nitrate-reducing hyperthermophilic (T(opt) = 100 degrees C) crenarchaeon. Clues were found suggesting explanations of the organism's surprising intolerance to sulfur, which may aid in the development of methods for genetic studies of the organism. Many interesting features wo...

The crystal structure of the oxidized form of flavodoxin from Desulfovibrio vulgaris has been studied at 2.0-A resolution, and a detailed description of the region around the flavin mononucleotide binding site is now available. The flavin is between a tyrosine group, roughly parallel to it on one side, and a tryptophan, about 45 degrees from being parallel, on the other side. The two carbonyl g...

Flavodoxin isolated from the blue-green alga, Anmyatis nidulana, crystallizes from ammonium sulfate in space group P2,2,2,, wwith a = 67.08 A, b = 69.24 A and c = 45.55 A. The diffraction patterns extend to a resolution of at least 1.8 A. Reduction of the flavin mononucleotide in the crystalline protein, to either the semi-quinone or fully reduced (hydroquinone) state, results in minimal change...

The riboflavin analog roseoflavin is an antibiotic produced by Streptomyces davawensis. Riboflavin transporters are responsible for roseoflavin uptake by target cells. Roseoflavin is converted to the flavin mononucleotide (FMN) analog roseoflavin mononucleotide (RoFMN) by flavokinase and to the flavin adenine dinucleotide (FAD) analog roseoflavin adenine dinucleotide (RoFAD) by FAD synthetase. ...

The sequence of a stretch of nucleotides affects its propensity for errors during replication and expression. Are proteins encoded by stable or unstable nucleotide sequences? If selection for variability is prevalent, one could expect an excess of unstable sequences. Alternatively, if selection against targets for errors were substantial, an excess of stable sequences would be expected. We scre...

Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster. In order to gain further insight into the function and properties of ISF, the...