Although microarray and expressed sequence tag (EST)-based approaches have been used to profile gene expression during baculovirus infection, the response of host genes to baculovirus infection and the interaction between baculovirus and its host remain largely unknown. To determine the host response to Bombyx mori nucleopolyhedrovirus infection and the dynamic interaction between the virus and...

Baculoviruses have evolved to control the defense mechanism of host cells and to replicate in the nuclei of infected cells efficiently. However, little is known about the dynamics and mechanism of baculovirus replication. To elucidate this, we have investigated the function of baculovirus genes and the baculovirus-host interaction using Bombyx mori nucleopolyhedrovirus (BmNPV). Here, we describ...

BACKGROUND Both tumor cells and their supporting endothelial cells should be considered for targeted cell killing when designing cancer treatments. Here we investigated the feasibility of combining radioiodide and antiangiogenic therapies after baculovirus-mediated transfer of genes encoding the sodium iodide symporter (NIS) and plasminogen kringle 5 (K5). METHODS A recombinant baculovirus co...

Influenza virus-like particles are currently evaluated in clinical trials as vaccine candidates for influenza viruses. Most commonly they are produced in baculovirus- or mammalian- expression systems. Here we used different vaccination schemes in order to systematically compare virus-like particle preparations generated in the two systems. Our work shows significant differences in immunogenicit...

The objective of this study was to prepare a copper-coated rubber surface using cold spray technology with improved virucidal and antimicrobial properties fight against highly transmissible viruses bacteria. A successful coating produced irregular-shaped pure Cu powder on an escalator handrail rubber. particles the deposited coatings (single double pass) were characterized in terms particle mor...

Black tiger shrimp, Penaeus monodon (Fabricius) is a non-piscine organism becomes most important aquatic resource of Bangladesh in the last decades. Traditionally it cultured 15-30 ppt salinity. Effect different low salinity on its survival, growth, production and economic return was observed three levels 10 ppt, 7 5 for 110 days. Juvenile shrimps (approximately 5g each) were stocked at 4 m-2 d...

Yellow head virus infects cultured shrimps and causes severe mortality resulting in a great economic loss. Haemolymph injection of dsRNA(pro) corresponding to the protease motif of YHV genome resulted in a complete inhibition of YHV replication. The effect of dsRNA lasted for at least 5 days. Injecting sequence-unrelated dsRNA(gfp) or dsRNA(TSV-pol) also resulted in an inhibition of YHV replica...

An improved polyhedrin-based baculovirus expression vector was constructed to expedite distinguishing infections by putative baculovirus recombinants from infections by wild-type (wt) baculovirus. The vector utilizes the Escherichia coli beta-galactosidase gene (lacZ) as a genetic marker for positive recombination between wt Autographa californica nuclear polyhedrosis virus and the baculovirus ...

1.Barsoum, J., R. Brown, M. McKee and F.M. Boyce. 1997. Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein. Hum. Gene Ther. 8:2011-2018. 2.Bowles, N.E., R.C. Eisensmith, R. Mohuiddin, M. Pyron and S.L.C. Woo. 1996. A simple and efficient method for the concentration and purification of recombinant retrovirus for increased ...