Introduction: Anthroponotic cutaneous leishmaniasis (ACL) is still a major public health problem in the northeast and central parts of Iran. This study was designed to compare microscopy and cultivation methods with PCR amplification of kinetoplast DNA and ITS1 followed by RFLP analysis for diagnosis of acute and chronic ACL. Methods: In this study, 66 patients with ACL including 24 acute and 4...

Molecular genetic testing is a reliable method for the definitive diagnosis of polycythemia vera (PV). The use interferon preparations in addition to hydroxyurea and antiplatelet agents could be associated with better therapeutic outcomes. In this report, we present clinical case young patient asymptomatic PV demonstrate effectiveness molecular analysis diagnosis.

introduction: anthroponotic cutaneous leishmaniasis (acl) is still a major public health problem in the northeast and central parts of iran. this study was designed to compare microscopy and cultivation methods with pcr amplification of kinetoplast dna and its1 followed by rflp analysis for diagnosis of acute and chronic acl. methods: in this study, 66 patients with acl including 24 acute and 4...

objective: classic cell culture and karyotyping is routinely used for prenatal detection of different chromosomal abnormalities. molecular cytogenetic techniques have also recently been developed and used for this purpose. quantitative florescence pcr using short tandem repeat (str) markers has more potential for high throughput diagnosis. marker heterozygosity in short tandem repeats (str) is ...

Fibro osseous lesions (FOLs) are a generic designation of poorly defined group of lesions which are recognized to affect the jaws and the craniofacial bones which are known for their confusing area in diagnostic pathology. The disease comprises of varied processes in which the normal architecture of bone is replaced by fibrous tissue containing varying amount of foci of mineralization. Regardle...

Turner's syndrome is a common disorder which occurs in around 1/3000 live births in girls. Diagnostic use of polymorphic DNA markers for the X chromosome could help to reduce the number of time consuming karyotype analyses needed. The M27 beta probe maps on the X chromosome to Xcen-Xp11-22 and in 83% of female subjects detects heterozygosity with multiallelic polymorphism. In Southern blotting,...

Pollen allergens are one of the main causes of type I allergies affecting up to 30% of the population in industrialized countries. Climatic changes affect the duration and intensity of pollen seasons and may together with pollution contribute to increased incidences of respiratory allergy and asthma. Allergenic grasses, trees, and weeds often present similar habitats and flowering periods compr...

Molecular biology-based assays are invaluable tools for the management of chronic viral hepatitis. They can be used to test blood donations, diagnose active infection, help to establish the prognosis, guide treatment decisions, and assess the virological response to therapy. This article reviews current molecular biology-based techniques and assays, and their practical use in the management of ...

circulating tumor cells (ctcs) recognition and characterization in the peripheral blood of patients with breast cancer have proven practical and predictive value in different studies. however, the clinical significance of ctcs enumeration and molecular characterization in thepersonalization of breast cancer diagnosis and treatment remains under the debate. a literature search in pubmed, web of ...