This review provides a critical appraisal of two of the more signiŽ cant contributions of the Minnesota approach to moral development. One contribution is the componential model which describes the four psychological components underlying moral behaviour. Evaluation of this model focuses on the adequacy of its synthesis of disparate processes in moral functioning, its instruments for assessing ...

The moral nature and ideals of Machiavelli, as portrayed in his work The Prince, have long been discussed and debated. Machiavelli has been characterized to such extremes as to be considered both the embodiment of political treachery and a civic visionary. The focus of this analysis is to investigate the moral considerations of Machiavelli’s model prince and to examine the image that these qual...

Does unethical behavior always represent selfish behavior? Consider the seminal dilemma that serves as the foundation for modern moral psychology: The wife of a man named Heinz was near death and desperate for a medicine that he could not afford (Kohlberg, 1963). To save her life, Heinz broke into a drugstore to steal the medicine. Kohlberg asked his subjects, “Should Heinz have stolen for his ...

The polar flagellum of Vibrio alginolyticus is rotated by the sodium motor. The stator unit of the sodium motor consists of four different proteins: PomA, PomB, MotX and MotY. MotX and MotY, which are unique components of the sodium motor, form the T-ring structure attached to the LP ring in the periplasmic space. MotY has a putative peptidoglycan-binding motif in its C-terminal region and MotX...

The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SSGCID Protein Production Group at the University of Washington (UW-PPG) has developed a high-throu...

BACKGROUND Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these proteins in a parallel, time and cost effective manner. RESULTS We developed a method for parallel expression and purification of recombinant proteins with a hexahistidine tag (H...

A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...