نتایج جستجو برای: moei bsa

تعداد نتایج: 6235  

Journal: :Optometry and vision science : official publication of the American Academy of Optometry 2007
Doerte Luensmann Mary-Ann Glasier Feng Zhang Vladimir Bantseev Trefford Simpson Lyndon Jones

PURPOSE To develop a novel in vitro method to detect the depth of penetration of the tear film protein albumin into contact lens materials using confocal laser scanning microscopy (CLSM). METHODS A poly-HEMA-based hydrogel (etafilcon A) and a silicone hydrogel material (lotrafilcon B) were examined. In vitro, bovine serum albumin (BSA) was labeled with 5-(4,6-dichloro-s-triazin-2-ylamino) flu...

2017
Yiwei Zhou Xin Fang Yu Gong Aiping Xiao Yixi Xie Liangliang Liu Yi Cao

BACKGROUND Nanoparticles (NPs) entering the biological environment could interact with biomolecules, but little is known about the interaction between unsaturated fatty acids (UFA) and NPs. METHODS This study used α-linolenic acid (LNA) complexed to bovine serum albumin (BSA) for UFA and HepG2 cells for hepatocytes. The interactions between BSA or LNA and ZnO NPs were studied. RESULTS The p...

2017
Tadashi Itoh Mifumi Kawabe Takahiko Nagase Hisami Matsushita Masami Kato Masafumi Miyoshi Kazuro Miyahara

The use of miniature pigs in non-clinical studies for medical drugs or devices has gradually been increasing in recent years. It is anticipated that the use of juvenile miniature pigs in laboratory practice will also increase. Therefore, it is important to investigate various parameters of juvenile miniature pigs. The body surface area (BSA) of an organism is one of the important parameters for...

Journal: :Biochimica et biophysica acta 2007
Kwanghun Chung Juhan Kim Byung-Kwan Cho Byoung-Joon Ko Bum-Yeol Hwang Byung-Gee Kim

The effect of dextran sulfate on protein aggregation was investigated to provide the clues of its biochemical mechanism. The interaction between dextran sulfate and BSA varied with the pH values of the solution, which led to the different extent of aggregation prevention by dextran sulfate. Light scattering data with thermal scan showed that dextran sulfate suppressed BSA aggregation at pH 5.1 ...

Journal: :Journal of lipid research 1988
F Martínez S Eschegoyen R Briones A Cuellar

In this study, we report a new method for cholesterol incorporation in mitochondria. This method is based on the coupling of cholesterol to bovine serum albumin (BSA) to form a structure with a spherical shape (designated C-BSA-C). The results show that C-BSA-C induces a specific cholesterol incorporation in mitochondria without interference from BSA. The cholesterol increase is complete in 1 m...

Journal: :The Journal of pharmacology and experimental therapeutics 1997
J F Ewing D V Young D R Janero D S Garvey T A Grinnell

Although nitrosothiols have been suggested to act as regulators of cell (patho)physiology, little is known about the pharmacology of nitrosylated proteins as nitric oxide (NO.) congeners. We describe the molecular consequences of nitrosylating bovine serum albumin (BSA) at multiple specific sites and demonstrate that the product S-nitrosoproteins exert NO.-like activity. The content of nucleoph...

Journal: :Journal of applied physiology 2005
Andrew D Baines Patrick Ho

Hypothetically either decreased nitric oxide (NO) or increased O(2) could initiate 20-HETE-mediated vasoconstriction associated with hemoglobin-based blood substitutes (HBOC). To test this hypothesis, we infused Tm-Hb, an HBOC with low O(2) affinity, into isoflurane-anesthetized Wistar (W) and Sprague-Dawley (SD) rats after exchanging 20% of their blood with Ringer lactate. For comparison we in...

Journal: :ACS applied materials & interfaces 2014
Ericka N J Ford Nisaraporn Suthiwangcharoen Paola A D'Angelo Ramanathan Nagarajan

Electrospun membranes were studied for the chemical deactivation of threat agents by means of enzymatic proteins. Protein loading and the surface chemistry of hybrid nanofibers influenced the efficacy by which embedded enzymes could digest the substrate of interest. Bovine serum albumin (BSA), selected as a model protein, was electrospun into biologically active fibers of poly(vinyl alcohol), P...

Journal: :The Journal of biological chemistry 2015
Hong-Zheng Meng Wei-Lin Zhang Fei Liu Mao-Wei Yang

The interaction between advanced glycation end products (AGEs) and receptor of AGEs (RAGE) is associated with the development and progression of diabetes-associated osteoporosis, but the mechanisms involved are still poorly understood. In this study, we found that AGE-modified bovine serum albumin (AGE-BSA) induced a biphasic effect on the viability of hFOB1.19 cells; cell proliferation was sti...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1995
S C Huang V D Talkad K P Fortune S Jonnalagadda C Severi G Delle Fave J D Gardner

We found that a variety of cholecystokinin (CCK) receptor ligands bind to bovine serum albumin (BSA). This binding was rapid, fully reversible, temperature independent, of low affinity, and specific for BSA; it depended on the concentration of BSA, the chemical structure of the ligand, and the chemical composition of the incubation medium. BSA also decreased the binding of 125I-labeled CCK octa...

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